Efficacy of Trichoderma asperellum for controlling Colletotrichum gloeosporioides causing anthracnose disease of Mangifera indica L. cv Nam Dok Mai
Article Sidebar
Main Article Content
Abstract
The efficacy test of the fungus Trichoderma asperellum in controlling anthracnose disease of water apple, caused by the fungus Colletotrichum gloeosporioides, was conducted by isolating the disease-causing fungus from mango leaves using the tissue transplanting method. A total of 20 isolates were tested for pathogenicity using the detached leaves method. It was found that isolate M05 induced the most severe disease symptoms. In assessing the effectiveness of T. asperellum in inhibiting the growth of C. gloeosporioides hyphae, the bi-culture method revealed that *T. asperellum* could inhibit hyphal growth and spore production by C. gloeosporioides* by 86.39 % and 78.87 %, respectively. Additionally, T. asperellum effectively controlled anthracnose on mango leaves in a laboratory greenhouse, achieving a 77.60 % reduction. However, no statistically significant difference was observed compared to T. harzianum (the Department of Agriculture strain) (p < 0.05).
Article Details
References
Chiangsin, R., Wanichkul, K., Guest, D., & Sangchote, S. (2016). Infection process of Colletotrichum gloeosporioides, the causal agent of anthracnose disease on mango leaf cv. Nam Dok Mai See Thong and chemical control. Songklanakarin Journal of Plant Science, 3(4), 49-55. (in Thai)
De los Santos-Villalobos, S., Guzmán-Ortiz, D. A., Gómez-Lim, M. A., Délano-Frier, J. P., de-Folter, S., Sánchez-García, P., & Peña-Cabriales, J. J. (2013). Potential use of Trichoderma asperellum (Samuels, Liechfeldt et Nirenberg) T8a as a biological control agent against anthracnose in mango (Mangifera indica L.). Biological Control, 64(1), 37-44. doi: 10.1016/j.biocontrol.2012.10.006
Department of Agricultural Extension. (2022). Production of quality mangoes for export. Bangkok, Thailand: Department of Agricultural Extension. (in Thai)
Intana, W., Promwee, A., & Yenjit, P. (2016). Using combination of wild type and mutant strains of Trichoderma asperellum for increasing the efficiency to control damping-off disease of tomato. Agricultural Science Journal, 47(3), 351-362. (in Thai)
Kaewchai, S. (2012). Application of Trichoderma spp. for plant disease control. Princess of Naradhiwas University Journal, 4(3), 108-123. (in Thai)
Leamkheng, S., Pinkeaw, C., & Jaijuathuet, P. (2018). Pathogen causing of postharvest disease in Jerusalem artichoke (Helianthus tuberosus L.) and in vitro control. Khon Kaen Agriculture Journal, 46(Suppl. 1), 1122-1129. (in Thai)
Na Nan, S., Jaipin, P., Jarintorn, S., & Klongkaew, B. (2017). Efficacy of Trichoderma harzianum on inhibition of the causal agent of Pummelo Tan Spot disease (Phyllosticta citriasiana). Thai Agricultural Research Journal, 35(3), 321-334. (in Thai)
Nuchnuanrat P., & Jitsatta, N. (2021). Evaluation of fungicide carbendazim resistance in Colletotrichum gloeosporioides causing anthracnose disease of mango cv. Aokrong in Chanthaburi province. RMUTSB Academic Journal, 9(2), 164-173. (in Thai)
Office of Agricultural Economics. (2021). Export statistics of fresh or frozen mangoes. Accessed June 1, 2024. Retrieved from http://impexp.oae.go.th/service/export.php. (in Thai)
Ratanacherdchai, K., Chaiyarak, T., & Juntachum, N. (2019). Preliminary investigation of Trichoderma asperellum’s culture and extracts against black rot of orchid. Prawarun Agricultural Journal, 16(1), 204-211. (in Thai)
Ratanacherdchai, K., Chaiyarak, T., & Juntachum, N. (2018). Preliminary investigation of antagonistic properties of Gliocladium sp. against chilli anthracnose disease caused by Colletotrichum acutatum. Proceeding of the 7th international conference on sciences and social sciences 2017: “Innovation research to stability, prosperity and sustainability” (pp. 72-78). Maha Sarakham, Thailand: Rajabhat Maha Sarakham University. (in Thai)
Rodriguez, R., & Redman, R. (2008). More than 400 million years of evolution and some plants still can’t make it on their own: plant stress tolerance via fungal symbiosis. Journal of Experimental Botany, 59(5), 1109-1114. doi: 10.1093/jxb/erm342
Somrug, K., & Teanglum, A. (2016). Efficacy of Trichoderma harzianum for biological control of white rust disease caused by Albugo ipomoeae-aquaticae in swamp morning glory. Prawarun Agricultural Journal, 13(1), 26-33. (in Thai)
Sukkhang, S. (2017). Biological control of plant pest. Accessed November 10, 2018. Retrieved from http://biology.ipst.ac.th/?p=3341. (in Thai)
Zapata-Sarmiento, D. H., Palacios-Pala, E. F., Rodríguez-Hernández, A. A., Melchor, D. L. M., Rodríguez-Monroy, M., & Sepúlveda-Jiménez, G. (2020). Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.). Biological Control, 140, 104105. doi: 10.1016/j.biocontrol.2019.104105
