Development of native chicken semen diluent for Thai native chicken breeds conservation and development
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Abstract
The objective of this study was to investigate the effect of KSU diluent (developed diluent), BPSE, Lake’s diluent and control (0.90 % NaCl) on the quality of native chicken sperm during cool storage from day 1 to day 4. The result for fresh semen of native chicken “Leung Hang Kao” showed sperm volume, sperm concentration, pH, osmolality, motility rate and viability rate were 0.39±0.04 ml/head, 2.28±0.13 x 109 sperm/ml, 7.38±0.02, 324±2.65 mOsm/kg, 91.67±14.43 % and 96.00±1.00 % respectively. The effect of KSU diluent, BPSE, Lake’s diluent and control on the diluted sperm quality of native chickens indicated that motility rate, progressive rate, average path velocity (VAP), curvilinear velocity (VCL), straight line velocity (VSL), viability rate, fertility rate and hatchability rate decreased with increasing storage time (P < 0.05). Sperm diluted with KSU diluent showed decreased motility rate, progressive rate, VAP, VCL and VSL by day 3 of storage (P < 0.05). However, sperm diluted with KSU diluent on days 1 and 2 higher fertility rates compared to BPSE and Lake’s diluent, though not significantly (P > 0.05). KSU diluent yielded the highest fertility rate (57.51±11.54 %) by day 3 of the storage (P < 0.05). Additionally, sperm diluted with KSU diluent, BPSE and Lake’s diluent did not significantly affect hatchability rates during storage (P > 0.05). Therefore, KSU diluent can be used to preserve native chicken semen for up to 3 days.
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References
Boonmatan, T. (2013). Cold and frozen storage of native chicken “Leung Hang Kao” semen. (Master’s thesis). Nakhon Ratchasima Thailand: Suranaree University of Technology.
Chalah, T., Seigneurin, F., Blesbois, E., & Brillard, J. P. (1999). In vitro comparison of fowl sperm viability in ejaculates frozen by three different techniques and relationship with subsequent fertility in vivo. Cryobiology, 39(2), 185-191. doi: 10.1006/cryo.1999.2201
Chankitisakul, V., Boonkum, W., Kaewkanha, T., Pimprasert, M., Ratchamak, R., Authaida, S., & Thananurak, P. (2022). Fertilizing ability and survivability of rooster sperm diluted with a novel semen extender supplemented with serine for practical use on smallholder farms. Poultry Science, 101(12), 102188. doi: 10.1016/j.psj.2022.102188
Chotesangasa, R. (2001). Effects of mating ratio, cock number in the flock and breeder age on fertility in Thai native chicken flock. Agriculture and Natural Resources, 35(2), 122 – 131.
Donoghue, A. M., & Wishart, G. J. (2000). Storage of poultry semen. Animal Reproduction Science, 62(1-3), 213-232. doi: 10.1016/S0378-4320(00)00160-3
Getachew, T., Lemma, A., & Goshu, G (2023). Effects of using commercial and homemade extenders on sperm quality of liquid stored semen of Horro chicken breed. Journal of World’s Poultry Research, 13(2), 216-222. doi: 10.36380/jwpr.2023.24
Guest, W. C. (1973). Spermatology and sperm preservation of channel catfish, Ictalurus punctatus. (Master’s thesis). Louisiana, United States: Louisiana State University.
Khaeruddin, K., Ciptadi, G., Yusuf, M., Hermawansyah, S., & Wahjuningsih, S. (2023). Effect of addition of glucose or fructose in three types of crystalloid fluid on spermatozoa quality of Gaga’ chicken from south Sulawesi, Indonesia. Pakistan Journal of Zoology, 1-8. doi: 10.17582/journal.pjz/20211128011130
Laopaiboon, B., & Duangjinda, M. (2012). Native chickens of Thailand: the past, present, and future. Khon Kaen Agriculture Journal, 40, 309-312. (in Thai)
Moreno, J. S., & Blesbois, E. (2020). Functional aspects of seminal plasma in bird reproduction. International Journal of Molecular Sciences, 21(16), 5664. doi:10.3390/ijms21165664
Phornwisetsirikun, S., Yano, T., Chotinun, S., & Rojanasthien, S. (2012). Knowledge, participation and opinion to operate model development project of rearing and management to reduce the risk of Avian Influenza and other outbreaks in native chicken of demonstration farm network farmers in the single outbreak and re-outbreak areas of Avian Influenza in Northern Thailand. Journal of Kasetsart Veterinarians, 22(2), 60-75. (in Thai)
Quinn, J. P., & Burrows, W. H. (1936). Artificial insemination in fowls. Journal of Heredity, 28, 31-37.
Sexton, T. J., & Fewlass, T. A. (1978). A new poultry semen extender 2. effect of the diluent components on the fertilizing capacity of chicken semen stored at 5oC. Poultry Science, 57(1), 277-284. doi: 10.3382/ps.0570277
Siudzinska, A., & Lukaszewicz, E. (2008). Effect of semen extenders and storage time on sperm morphology of four chicken breeds. Journal of Applied Poultry Research, 17(1), 101- 108. doi: 10.3382/japr.2007-00048
