Efficacy of Streptomyces sp. Isolate SRF1 to Control of the Fungus Pestalotia spp., Causal Agent of Mango Leaf Spot

Abstract

Pestalotia spp., is a causal agent of leaf spot of many fruits and economic crops, especially mango. In addition, this fungal pathogen caused production yield losses. Therefore, the purpose of this study was to evaluate the antifungal efficacy of Streptomyces sp. SRF1 against 10 isolates of Pestalotia spp. isolated from mango leaf spot disease using dual culture method. The results showed that Streptomyces sp. SRF1 could inhibit the mycelial growth of all tested fungi in range 73.37-91.51%. The 21 days culture filtrate of Streptomyces sp. SRF1 in arginine was also used to determine the antifungal activity by agar well diffusion assay. The results revealed that the culture filtrate from arginine media had the antifungal activity with 39.34-64.36% reduction growth. Moreover, the culture filtrates was used to evaluate the fungal mycelial growth and spore germination. The results revealed that the culture filtrate had also inhibited fungal mycelial growth and spore germination. In addition, abnormal mycelial and short germ tubes of spore were observed when compared with the control treatment.