ตัวชี้วัดความเครียดออกซิเดชันในเซลล์เยื่อบุเต้านมที่แยกได้จากน้ำนมแพะ
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เผยแพร่แล้ว:
ส.ค. 13, 2021
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ความเครียดออกซิเดชัน เซลล์เยื่อบุเต้านม น้ำนมแพะ oxidative stress mammary epithelial cells goat milk
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บทคัดย่อ
วัตถุประสงค์ของการศึกษานี้เพื่อวัดระดับการเกิดความเครียดออกซิเดชันในตัวสัตว์และเซลล์เยื่อบุเต้านมที่แยกได้จากน้ำนมแพะ แบ่งแม่แพะนมพันธุ์ซาเนนเป็น 3 กลุ่มตามเดือนที่ 1, 2 และ 3 ของระยะการให้นม การเก็บตัวอย่างเลือดและเซลล์จากน้ำนมเพื่อตรวจวัดความเข้มข้นของสาร malondialdehyde (MDA) และกิจกรรมของเอนไซม์ glutathione peroxidase (GPx) ผลการทดลองพบว่าแพะกลุ่มที่ 2 มีความเข้มข้นของ MDA และกิจกรรมของเอนไซม์ GPx ในพลาสม่าสูงกว่ากลุ่มที่1 และ 3 อย่างมีนัยสำคัญ ในทางกลับกันความเข้มข้นของ MDA และกิจกรรมของเอนไซม์ GPx ในเซลล์เยื่อบุเต้านมที่แยกได้จากน้ำนมแพะของกลุ่มทดลองไม่มีความแตกต่างอย่างมีนัยสำคัญ สรุปได้ว่าความเครียดออกซิเดชันในแพะระหว่างการให้นมสามารถเกิดขึ้นได้ทั้งในระดับตัวสัตว์ และในเต้านม นอกจากนี้เซลล์เยื่อบุเต้านมที่แยกได้จากน้ำนมแพะสามารถนำมาใช้วิเคราะห์และบ่งชี้สถานะความเครียดออกซิเดชันในเต้านมได้ อย่างไรก็ตามเซลล์เยื่อบุเต้านมที่แยกได้จากน้ำนมแพะไม่สามารถใช้เป็นตัวแทนเพื่อเป็นตัวบ่งชี้การเกิดความเครียดออกซิเดชันในระดับตัวสัตว์ และไม่มีสหสัมพันธ์กับสถานะความเครียดออกซิเดชันในพลาสม่า
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References
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2. Castillo C, Hernández J, Valverde I, Pereira V, Sotillo J, Alonso ML, et al. Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Res Vet Sci 2006 Aug 9;80(2):133-9.
3. Hall DM, Buettner GR, Matthes RD, Gisolfi CV. Hyperthermia stimulates nitric oxide formation: electron paramagnetic resonance detection of NO-heme in blood. J Appl Physio 1994;77(2) :548-53.
4. Nunomura A, Castellani RJ, Zhu X, Moreira PI, Perry G, Smith MA. Involvement of Oxidative Stress in Alzheimer Disease. J Neuropathol Exp Neurol 2006 Jul 01;65(7):631-41.
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6. Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol Review 1979;59(3):527-605.
7. Knight CH, Hillerton JE, Teverson RM, Winter A. Biopsy of the bovine mammary gland. British Vet J 1992 Jun 19;148(2):129-32.
8. Taylor-Papadimitriou J, Shearer M, Tilly R. Some properties of cells cultured from early-lactation human milk. J Natl Cancer Inst. 1977Jun 01;58(6):1563-71.
9. Sigl T, Meyer H, Wiedemann S. Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation. Czech J Anim Sci 2012 May 22;57(10):469-80.
10. Boutinaud M, Rulquin H, Keisler DH, Djiane J, Jammes H. Use of somatic cells from goat milk for dynamic studies of gene expression in the mammary gland. J Anim Sci 2002;80(5):1258-69.
11. Hu H, Zhang Y, Zheng N, Cheng J, Wang J. The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells. Anim Sci J 2016 Oct 15 5;87(1):84-91.
12. Peng X, Lu L, Li Y, Yan P. Mammary Cell Turnover under High Temperature during the Dry Period in Dairy Cows. Asian-Australasian J Anim Sci 2011 Dec 8;24(4):485-92.
13. Tao S, Bubolz JW, do Amaral BC, Thompson IM, Hayen MJ, Johnson SE, et al. Effect of heat stress during the dry period on mammary gland development. J Dairy Sci 2011 Sep 16;94(12): 5976-86.
14. Abuelo A, Hernández J, Benedito JL, Castillo C. Redox Biology in Transition Periods of Dairy Cattle: Role in the Health of Periparturient and Neonatal Animals. Antioxidants (Basel, Switzer- land) 2019 Jan 13;8(1):doi:10.3390/antiox8010 020.
15. Kenyon PR, Maloney SK, Blache D. Review of sheep body condition score in relation to production characteristics. New Zeal J Agr Res 2014 Feb 10; 57(1):38-64.
16. Putman AK, Brown JL, Gandy JC, Wisnieski L, Sordillo LM. Changes in biomarkers of nutrient metabolism, inflammation, and oxidative stress in dairy cows during the transition into the early dry period. J Dairy Sci 2018 Aug 01;101(10):9350-9.
17. Kapusta A, Kuczyńska B, Puppel K. Relationship between the degree of antioxidant protection and the level of malondialdehyde in high-performance Polish Holstein-Friesian cows in peak of lactation. PLoS One 2018 Mar 1;13(3): e01935 12.
18. Miyamoto Y, Koh YH, Park YS, Fujiwara N, Sakiyama H, Misonou Y, et al. Oxidative stress caused by inactivation of glutathione peroxidase and adaptive responses. Biol Chem 2003;384(4): 567-74.
19. Celi P. The role of oxidative stress in small ruminants' health and production. R Bras Zootec 2010;39:348-63.
20. 20.Chaiyabutr N, Chanchai W, Boonsanit D, Sitprija S, Chanpongsang S. Different Responses of Oxidative Stress Index in the Plasma of Crossbred Holstein Cattle During Cooling and Supplemental Recombinant Bovine Somatotropin. J Anim Vet Adv 2011;10(8):1045-53.
21. Mohebbi-Fani M, Ansari-Lari M, Nazifi S, Abbasi F, Shabbooei Z. Oxidative Status and Acute Phase Response in Post-transition Early- and Mid-lactation Holstein Cows and Their Correlations with Some Performance Records. J Fac Vet Med Istanbul Univ 2016;42:DOI:10.16988/iuvfd.2016.55 237.
22. Abeni F, Calamari L, Stefanini L. Metabolic conditions of lactating Friesian cows during the hot season in the Po valley. 1. Blood indicators of heat stress. Int J Biometeorol 2007 May 9;52(2):87-96.
23. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemo- cuprein). J Biol Chem 1969 Nov 25;244(22):6049 -55.
24. Moolchandani A. A Review: Oxidative Stress during Lactation in Dairy Cattle. Dairy Vet Sci J 2018 Apr 04;5:DOI:10.19080 /JDVS. 2018.05.5556 69.
25. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clinl Med 1963 Apr 30;61:882-8.
26. McDowell LR, Arthington JD. Minerals for grazing ruminants in tropical regions. 4th Gainesville: Institute of Food and Agricultural Sciences, University of Florida; 2005. 86 p.p.
27. Spitz DR, Li GC, McCormick ML, Sun Y, Oberley LW. Stable H2O2-resistant variants of Chinese hamster fibroblasts demonstrate increases in catalase activity. Radiat Res 1988;114(1):114-24.
28. Strålin P, Marklund SL. Effects of oxidative stress on expression of extracellular superoxide dismutase, CuZn-superoxide dismutase and Mn-superoxide dismutase in human dermal fibroblasts. Biochem J 1994 Mar 01;298 (Pt 2): 347-52.
29. Gille JJ, Joenje H. Cell culture models for oxidative stress: superoxide and hydrogen peroxide versus normobaric hyperoxia. Mutat Res 1992;275(3-6):405-14.
2. Castillo C, Hernández J, Valverde I, Pereira V, Sotillo J, Alonso ML, et al. Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Res Vet Sci 2006 Aug 9;80(2):133-9.
3. Hall DM, Buettner GR, Matthes RD, Gisolfi CV. Hyperthermia stimulates nitric oxide formation: electron paramagnetic resonance detection of NO-heme in blood. J Appl Physio 1994;77(2) :548-53.
4. Nunomura A, Castellani RJ, Zhu X, Moreira PI, Perry G, Smith MA. Involvement of Oxidative Stress in Alzheimer Disease. J Neuropathol Exp Neurol 2006 Jul 01;65(7):631-41.
5. Patel VP, Chu CT. Nuclear transport, oxidative stress, and neurodegeneration. Int J Clin Exp Pathol 2011 Mar 31;4(3):215-29.
6. Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol Review 1979;59(3):527-605.
7. Knight CH, Hillerton JE, Teverson RM, Winter A. Biopsy of the bovine mammary gland. British Vet J 1992 Jun 19;148(2):129-32.
8. Taylor-Papadimitriou J, Shearer M, Tilly R. Some properties of cells cultured from early-lactation human milk. J Natl Cancer Inst. 1977Jun 01;58(6):1563-71.
9. Sigl T, Meyer H, Wiedemann S. Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation. Czech J Anim Sci 2012 May 22;57(10):469-80.
10. Boutinaud M, Rulquin H, Keisler DH, Djiane J, Jammes H. Use of somatic cells from goat milk for dynamic studies of gene expression in the mammary gland. J Anim Sci 2002;80(5):1258-69.
11. Hu H, Zhang Y, Zheng N, Cheng J, Wang J. The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells. Anim Sci J 2016 Oct 15 5;87(1):84-91.
12. Peng X, Lu L, Li Y, Yan P. Mammary Cell Turnover under High Temperature during the Dry Period in Dairy Cows. Asian-Australasian J Anim Sci 2011 Dec 8;24(4):485-92.
13. Tao S, Bubolz JW, do Amaral BC, Thompson IM, Hayen MJ, Johnson SE, et al. Effect of heat stress during the dry period on mammary gland development. J Dairy Sci 2011 Sep 16;94(12): 5976-86.
14. Abuelo A, Hernández J, Benedito JL, Castillo C. Redox Biology in Transition Periods of Dairy Cattle: Role in the Health of Periparturient and Neonatal Animals. Antioxidants (Basel, Switzer- land) 2019 Jan 13;8(1):doi:10.3390/antiox8010 020.
15. Kenyon PR, Maloney SK, Blache D. Review of sheep body condition score in relation to production characteristics. New Zeal J Agr Res 2014 Feb 10; 57(1):38-64.
16. Putman AK, Brown JL, Gandy JC, Wisnieski L, Sordillo LM. Changes in biomarkers of nutrient metabolism, inflammation, and oxidative stress in dairy cows during the transition into the early dry period. J Dairy Sci 2018 Aug 01;101(10):9350-9.
17. Kapusta A, Kuczyńska B, Puppel K. Relationship between the degree of antioxidant protection and the level of malondialdehyde in high-performance Polish Holstein-Friesian cows in peak of lactation. PLoS One 2018 Mar 1;13(3): e01935 12.
18. Miyamoto Y, Koh YH, Park YS, Fujiwara N, Sakiyama H, Misonou Y, et al. Oxidative stress caused by inactivation of glutathione peroxidase and adaptive responses. Biol Chem 2003;384(4): 567-74.
19. Celi P. The role of oxidative stress in small ruminants' health and production. R Bras Zootec 2010;39:348-63.
20. 20.Chaiyabutr N, Chanchai W, Boonsanit D, Sitprija S, Chanpongsang S. Different Responses of Oxidative Stress Index in the Plasma of Crossbred Holstein Cattle During Cooling and Supplemental Recombinant Bovine Somatotropin. J Anim Vet Adv 2011;10(8):1045-53.
21. Mohebbi-Fani M, Ansari-Lari M, Nazifi S, Abbasi F, Shabbooei Z. Oxidative Status and Acute Phase Response in Post-transition Early- and Mid-lactation Holstein Cows and Their Correlations with Some Performance Records. J Fac Vet Med Istanbul Univ 2016;42:DOI:10.16988/iuvfd.2016.55 237.
22. Abeni F, Calamari L, Stefanini L. Metabolic conditions of lactating Friesian cows during the hot season in the Po valley. 1. Blood indicators of heat stress. Int J Biometeorol 2007 May 9;52(2):87-96.
23. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemo- cuprein). J Biol Chem 1969 Nov 25;244(22):6049 -55.
24. Moolchandani A. A Review: Oxidative Stress during Lactation in Dairy Cattle. Dairy Vet Sci J 2018 Apr 04;5:DOI:10.19080 /JDVS. 2018.05.5556 69.
25. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clinl Med 1963 Apr 30;61:882-8.
26. McDowell LR, Arthington JD. Minerals for grazing ruminants in tropical regions. 4th Gainesville: Institute of Food and Agricultural Sciences, University of Florida; 2005. 86 p.p.
27. Spitz DR, Li GC, McCormick ML, Sun Y, Oberley LW. Stable H2O2-resistant variants of Chinese hamster fibroblasts demonstrate increases in catalase activity. Radiat Res 1988;114(1):114-24.
28. Strålin P, Marklund SL. Effects of oxidative stress on expression of extracellular superoxide dismutase, CuZn-superoxide dismutase and Mn-superoxide dismutase in human dermal fibroblasts. Biochem J 1994 Mar 01;298 (Pt 2): 347-52.
29. Gille JJ, Joenje H. Cell culture models for oxidative stress: superoxide and hydrogen peroxide versus normobaric hyperoxia. Mutat Res 1992;275(3-6):405-14.