Mutagenesis induction of Moniliella sp. BCC25224 using combination of UV and ethyl methanesulfonate (EMS) for erythritol production

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chamiporn suwanapetch
Wirat Vanichsriratana

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Erythritol is a safe, non-caloric and non-insulinemic sweetener present in various foods. It can be produced from glucose as substrate via fermentation with Moniliella pollinis (a safe and suitable food-grade osmophilic yeast). This study aims to screen high erythritol producing Moniliella sp. strains via mutagenesis. The candidate producer, Moniliella sp. BCC25224, was mutated sequentially by UV irradiation and immersion in ethyl methyl sulphonate (EMS) for 15, 30, and 60 min. The mutant named Moniliella sp. BCC25224-M13 was selected based on the highest levels of erythritol production at flask scale (44.2 gL-1) which was 1.2-fold improved compared with its wild parent. In a 10-L bioreactor, the erythritol production, erythritol yield and yield coefficients by BCC25224-M13 were 103.5 gL-1, 56.9% and 0.59 gg-1, respectively. This study revealed that Moniliella sp. BCC25224 could be used as a parent to produce mutants for superior erythritol production, such as Moniliella sp. BCC25224-M13.

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References

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Burschapers, J., Schustolla, D., Schugerl, K., Roper, H. & Troostembergh, JC.de. (2002). Engineering aspects of the production of sugar alcohols with the osmophilic yeast Moniliella tomentosa var pollinis Part 2. Batch and fed-batch operation in bubble column and airlift, Process Biochemistry, 38, 559- 570. DOI:10.1016/S0032-9592 (02) 00179-6