Strain improvement of Moniliella sp. BCC25224 using mutagenesis
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Abstract
This study aimed at screening high erythritol producing Moniliella sp strains via mutagenesis. The candidate producer, Moniliella sp. BCC25224, was mutated sequentially by UV irradiation and immersion in ethyl methyl sulphonate (EMS) at 15, 30, and 60 min. The mutant name Moniliella sp. BCC25224- M13 was selected for the highest erythritol production with erythritol yield and yield coefficients of 56.9% erythritol yield, and 0.59 gg-1 yield, respectively in a 10-L bioreactor. This study revealed that Moniliella sp. BCC25224 could be used as a parent to produce mutant for superior erythritol such as Moniliella sp. BCC25224- M13.
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References
Burschapers, J., Schustolla, D., Schugerl, K., Roper, H. & Troostembergh, JC.de. (2002). Engineering aspects of the production of sugar alcohols with the osmophilic yeast Moniliellatomentosa var pollinis. Part I. Batch and fed-batch operation in stirred tank. Process Biochemistry, 38, 497-506. DOI:10.1016/ S0032-9592 (02) 00178-4
Burschapers, J., Schustolla, D., Schugerl, K., Roper, H. & Troostembergh, JC.de. (2002). Engineering aspects of the production of sugar alcohols with the osmophilic yeast Moniliella tomentosa var pollinis Part 2. Batch and fed-batch operation in bubble column and airlift, Process Biochemistry, 38, 559- 570. DOI:10.1016/S0032-9592 (02) 00179-6
