Antioxidant activity of Carissa carandas Linn. fruit extract and its application in a liquid soap product

Abstract

Carissa carandas Linn. is a plant that has medicinal properties. Extracts from the fruit and leaves of this plant contain antioxidant active substances with high efficiency. The objectives of this research were to study the antioxidant activity of extracts of C. carandas L. fruit, investigate the effect of inhibiting the activity of the enzyme tyrosinase, and develop a liquid soap formula from extracts of C. carandas L.  fruit tested according to TIS standards 14–2019. The research results found that C. carandas L. fruit extract made from 95% ethanol has a viscous consistency. It has a dark red color and smells like burnt sugar. It has a yield of 21.80%by mass. Testing the antioxidant activity of C. carandas L. extract using the DPPH method compared to standard vitamin C. It has an IC₅₀ value equal to 14.24 + 0.03 mg/mL and tested the effect of inhibiting the activity of the tyrosinase enzyme using the modified dopachrome method. It was found that the inhibition value of the tyrosinase enzyme of the C. carandas L. extract was 50%, equal to 14.37 +0.05 mg/mL. When used to develop a liquid soap product from C. carandas L. fruit extract and test the chemical properties of the liquid soap from C. carandas L. fruit extract, it was found that it has a total fat content of 15.40 percent by mass. It has an acidity-alkalinity of 4.5. No free alkalinity was found. There is an amount of substances that are not soluble in ethanol of 0.45 percent by mass, which passes the TIS 14-2019 standard criteria. Antioxidants from C. carandas L. fruit are very interesting because the C. carandas L. fruit has properties that are beneficial to skin health and contains extracts that have antioxidant effects that can be used in dietary supplements and cosmetics.