Feasibility study on the utilization of phospholipids derived from para rubber seed oil for liposome particles formation
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Abstract
Liposome particles can be synthesized using phospholipids as precursors. These particles have the capability to encapsulate crucial substances, prevent degradation and enhance stability of the substances. The primary aim of this research is to investigate the application of phospholipids derived from para rubber seed oil for the formulation of liposome particles. Oil was extracted from para rubber seeds of the RRIM 600 variety using hexane as a solvent. Phospholipids were separated from the oil through a heat and water degumming process. The type and quantity of phospholipids were analyzed via high-performance liquid chromatography technique. Liposome particles were synthesized using the reverse phase evaporation method, and the physical properties of liposome particles were measured by using dynamic light scattering technique. The morphology of liposome particles was examined through transmission electron microscopy. Additionally, the stability of the liposome particles was examined over a period of 0 to 12 weeks at 4oC. The oil content in para rubber seeds was found to be 45.25±0.04 %w/w. The predominant phospholipids identified in the gums isolated from para rubber seed oil were phosphatidylcholine and phosphatidylethanolamine (22.36±3.10 and 17.39±0.51 mg/100g of oil). When synthesizing liposome particles using 0.1-2.0 %w/v of phospholipids, it was observed that a homogeneous yellow mixture was obtained, with particle dispersed in an aqueous phase. These liposomes exhibited a spherical shape, with sizes ranging from 524 to 644 nanometer. The polydispersity index ranged from 0.33 to 0.46. The z-potential of the particles ranged from -33 to -58 mV and the synthesized particles exhibited the highest stability for 8 weeks. In summary, the phospholipids derived from para rubber seed oil can be utilized to form liposome particles.
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