Efficiency of liquld media culbres on growth and development of Robiquetia succisa (Lindl.) Seidenf. & Garay
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Abstract
The study of efficiency of Murashige and Skoog (1962) liquid medium for in vitro culture of young seedlings of Robiquetia succisa (Lindl.) Seidenf. & Garay in static and shaking culture conditions, supplemented with 150 ml/l coconut water and various concentrations of 6-Benzylaminopurine (BA) and 1-Naphthalene acetic acid (NAA) (0, 0.5, 1.0, and 2.0 mg/l) for 12 weeks. The results indicated that culturing young seedlings in the liquid medium supplemented with 150 ml/l coconut water, using the static culture method, was the most effective in inducing the proliferation of protocorm-like bodies (PLBs) (more than 30 PLBs per explant in average) from the root segments of R. succisa seedlings. Additionally,
R. succisa plantlets developed without planting materials exhibited the highest survival rate of 90% compared to those grown with lump charcoal or sphagnum moss as planting materials when cultivated in a greenhouse for 12 weeks.
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References
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