Effects of NAA and activated charcoal on root induction of Impatiens sirindhorniae Triboun & Suksathan
Article Sidebar
Main Article Content
Abstract
Impatiens sirindhorniae are beautiful flowering plants and endemic plants that grow on limestone mountains. It was only found in Krabi province and Surat Thani province. Currently, its conservation status was classified as vulnerable species. Micropropagation allows the production of plantlet for preservation genetic resources. The objective of this research was to investigate the influence of NAA and activated charcoal on micro-propagation of I. sirindhorniae for producing plantlet for transplanting and conservation in their natural habitat. Shoots developed explant obtained from cultivation in sterile conditions, approximately 2.0 centimeters long and without root formation, were taken for root induction. Shoot explants were cultured on 1/3 strength of solidified MS medium, 2 shoots per bottle. The experiment was divided into 6 treatments viz. 1/3 MS supplemented with various concentrations of NAA (0, 0.5, 1.0, 1.5, and 2.0 mg/L) and 1 g/L of activated charcoal. The treatment with only 1/3 MS is control. After cultured for 4 weeks, the root induction rate, number of roots and root characteristics were recorded. Three replications, with ten bottles per replicate and two explants per bottle, were used for each treatment. CRD experiment in a completely randomized design was carried out. The root induction rate, root length and root width were highest in control (100%, 3.50 cm, 0.23 cm). However, plantlet cultured on media supplemented with activated charcoal (treatment 6) had root induction rate (100%), average number of roots per plant (5.58 roots) and root length (2.66 cm), not significantly different from the control. Therefore, it can be concluded that all concentrations of NAA had an inhibitory effect on root induction in I. sirindhorniae. While 1/3 MS supplemented with or without activated charcoal should be used on root induction in I. sirindhorniae.
Article Details
References
De Mello, T., De Oliveira, G.E., Alexandre, S.R., Schmildt, R.E., & Otoni, C.W. (2020). Establishment and in vitro morphogenesis of Sapucaya explants (Lecythidaceae). In Vitro Cellular & Developmental Biology, 56(6), 882–893.
Desai, P., Desai, S., Patel, A., Mankad, M., Gajera, B., Patil, G., & Narayanan, S. (2019). Development of efficient micropropagation protocol through axillary shoot proliferation for Bambusa vulgaris ‘wamin’ and Bambusa bambos and assessment of clonal fidelity of the micropropagated plants through Random Amplified Polymorphic DNA markers. Agriculture and Natural Resource, 53, 26–32.
Kaviani, B., Almasi, A., Sedaghathoor, S., & Khoddamzadeh, A.A. (2021). A protocol for fast in vitro propagation of Impatiens hawkeri cv. Sweeties Blue Star, an important ornamental-cover species. Rodriguésia, 72, e02562019.
Kongbonsook, P., Jarutsumrid, N., Saridnum, O., & Thanarut, C. (2021). Plant Growth Regulators on Melientha suavis Pierre Propagations. Naresuan Agricultural Journal, 18(2), e0180201.
Luo, L., Li, Y., Huang, X., Zhang, X., Tan, Y., Liu, Z., Huang, H. & Huang, M. (2023). Establishment of a highly efficient in vitro plant regeneration system for Impatiens hawkeri W. Bull cv. ‘Pink’ and its background resistance to kanamycin. Plant Cell, Tissue and Organ Culture, 155, 627–636.
Mohamed, N., Taha, R.M., Razak, U., & Elias, H. (2018). The Role of Plant Growth Regulators in the Development of in vitro Flowering, Histology and Ultrastructural Studies in Impatiens balsamina cv. Dwarf Bush. Planta Daninha, 36, e018165357.
Murashige, T., & Skoog, F. (1962). A reverse medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology, 15, 473-497.
Owen, H.R., Wengerd, D., & Miller, A.R. (1991). Culture medium pH is influenced by basal medium,carbohydrate source, gelling agent, activated charcoal, and medium storage method. Plant Cell Reports, 10, 583-586.
Pattarakulpisutti, P. (2021). Floral morphology and pollination readiness of Impatiens sirindhorniae Triboun & Suksathan in a greenhouse: a preliminary study for conservation. Thai Journal of Botany, 13(2), 81–91.
Promchan, T., Ramasoot, S., & Bunwest, P. (2016). Effects of Activated Charcoal and Growing Media on Seedling Growth and Survival Rate of Aeridesodorata. Wicha Journal, 35(2), 53–61.
Rakkid, P., Triboun, P., Sangkasa-ad, P., Piriyavinit, P., & Kanjanawattanawong, P. (2013). Study on in vitro Conservation Technique of Impatiens sirindhorniae and Zingiber sirindhorniae. In. Annual Academic Seminar 2013, Biotechnology Research and Development Office. Bangkok: Biotechnology Research and Development Office Department of Agriculture.
R Core Team. (2019). R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing.
Samala, S., Kongton, K., Yenchon, S., Petchsri, S., Suwannakong, Y., Rotchanajinda, W., & Chareonsap, P.P. (2022a). Effect of auxins and cytokinin on micropropagation of Impatiens sirindhorniae Triboun & Suksathan in vitro. Acta Horticulturae, 1339, 217–225.
Samala, S., Kongton, K., Yenchon, S., Petchsri, S., Suwannakong, Y., Rotchanajinda, W., Buakaew, P., Suleethat, N., Thepsuriwong, S., Pengjun, W., Nongkaew, S., Chareonsap, P.P. & Thammasiri, K. (2022b). Enhancement of surface sterilization protocol for in vitro propagation of Impatiens sirindhorniae. Acta Horticulturae, 1334, 257–262.
Senawong, R., Sansuk, P., & Sansuk, S. (2014). In vitro tissue culture of Dendrobium kontumense Gagnep. KKU Science Journal, 19, 399–413.
Suksathan, P., & Triboun, P. (2009). Ten new species of Impatiens (Balsaminaceae) from Thailand. Gardens’ Bulletin Singapore, 61(1), 159–184.
Te-chato, S., & Lim, M. (1999). Plant regeneration of mangosteen via nodular callus formation. Plant Cell, Tissue and Organ Culture, 59, 89–93.
Tharawoot, T., Samyam, W., & Vessabutr, S. (2012). Colchicine induced polyploidy of in vitro Impatiens patula Craib. Thai Journal of Botany, 4 (Special Issue), 75–80.
Thomas, D. (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26(6), 618–631.
Ziaratmai, S.M., Kunert, K.J., & Lall, N. (2009). Elicitation of 7-methyjuglone in Drosera capensis. South African Journal of Botany. 75, 97–103.
