Phylogroups and antimicrobial susceptibility profiles of Escherichia coli Isolated from urine of female patients with urinary tract infections in a tertiary hospital in Bangkok
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Abstract
Urinary tract infections (UTIs) caused by Escherichia coli are a global health concern, particularly among females. This cross-sectional study aimed to investigate and analyze the relationship between phylogroups and antimicrobial susceptibility profiles of E. coli isolates from female UTI patients. We analyzed 174 non-duplicate E. coli isolates obtained from urine samples of female UTI patients admitted to a tertiary hospital in Bangkok, Thailand, between November 2023 and January 2024. Antimicrobial susceptibilities of E.coli were performed using the disk diffusion method, and their phylogroups were determined using quadruplex PCR. Antimicrobial susceptibility test revealed that the resistance rate of E. coli to ampicillin, ciprofloxacin, and trimethoprim-sulfamethoxazole were 88.5, 63.8, and 51.1% respectively. Additionally, 36.8% of isolates were extended-spectrum beta-lactamase (ESBL) producers, and 61.3% were multidrug-resistant bacteria (MDR). The most prevalent phylogroup was B2 (61.5%), followed by D (9.2%), C (7.5%), B1 (7.5%), A (5.2%), F (4.0%), E (3.4%), and unknown (1.7%). Notably, phylogroup C exhibited significantly higher resistance rates to ceftazidime, ciprofloxacin, and gentamicin (p<0.05) and was the only phylogroup exhibiting carbapenem resistance. In contrast, phylogroup E showed a significantly higher prevalence of MDR. This study demonstrated that E. coli isolates from female UTI patients exhibited high antimicrobial resistance rates, reflecting the need for surveillance and control measures to prevent the spread of these drug-resistant strains.
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