ฤทธิ์ต้านจุลินทรีย์ ต้านอนุมูลอิสระ และยับยั้งเอนไซม์แอลฟากลูโคซิเดสของสารสกัดหยาบจากผลของบาร์บาโดสเชอร์รี (Malpighia glabra)
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Abstract
This research aims to evaluate the antimicrobial, antioxidant and anti-alpha-glucosidase activities of crude extracts from Malpighia glabra fruits. The fruits were extracted with hexane, dichloromethane and methanol. Three crude extracts were evaluated for their antimicrobial, antioxidant and alpha-glucosidase inhibitory activities by using a colorimetric broth microdilution test, DPPH free radical scavenging assay and alpha-glucosidase inhibitory activity assay, respectively. Six pathogenic bacteria, Staphylococcus aureus, methicillin resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Cryptococcus neoformans, were used in the antimicrobial test. Hexane extract was the most active against C. albicans with MIC and MFC of 8 of 16 µg/mL, respectively. The extract killed target microorganism by destroying cell surface. Dichloromethane extract had the strongest anti-alpha-glucosidase activity with IC50 value of 1.12 mg/mL. On the other hand, methanol extract had the strongest antioxidant activity with EC50 value of 1.15 mg/mL. These results showed that crude extracts from the M. glabra fruits are interesting because they are a good source of many bioactive compounds.
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