Histological characterization of Ensidens ingallsianus ingallsianus (Lea, 1852) from Songgaria River during Sangkhlaburi District, Kanchanaburi Province, Thailand

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Ezra Mongkolchaichana
Jes Kettratad
Natthakitt To-orn
Sinlapachai Senarat
Chanyut Sudtongkong
Wannee Jiraungkoorskul
Anan Kenthao

Abstract

Observation of the health status of the freshwater aquatic organisms in Thailand has been rarely reported. In the present study, the health status of Ensidens ingallsianus ingallsianus (Lea, 1852) from Songgaria River at Sangkhlaburi District, Thailand was investigated using histological techniques. The samples were obtained from two seasons, including cold (December 2015) and dry (April 2016) seasons. The analyzed specimens from each season were classified into three groups with ten samples based on the distribution of shell length, including 1-2, 3-4 and 6-8 cm. The specimens were then processed via the standard paraffin method and then stained with Harris' hematoxylin and eosin (H&E), periodic acid-schiff (PAS) and Masson’s trichrome (MT). Results from the study revealed that the histological appearances of skin, gill, esophagus, stomach and digestive gland of this bivalve were similar to those in other bivalves. Particularly, the samples with 1-2 cm of shell length exhibited the different structure of digestive glands with the blind-ending tubules. Each tubule was lined with digestive cell and basophilic cell containing  slight basophilic cytoplasm. A few histopathological observations of E. ingallsianus ingallsianus were observed, suggesting that this bivalve is considered to the good health status and lived in adequate environmental conditions of life in Songgaria River.

Article Details

How to Cite
Mongkolchaichana, E., Kettratad, J., To-orn, N., Senarat, S., Sudtongkong, C., Jiraungkoorskul, W., & Kenthao, A. (2021). Histological characterization of Ensidens ingallsianus ingallsianus (Lea, 1852) from Songgaria River during Sangkhlaburi District, Kanchanaburi Province, Thailand. YRU Journal of Science and Technology, 6(1), 37–45. retrieved from https://li01.tci-thaijo.org/index.php/yru_jst/article/view/241506
Section
Research Article

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