Comparison of Anti-inflammatory Effects of Centella asiatica (L.) Urb. Leaf Extract in Macrophages
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Abstract
Bua-bog (Centella asiatica (L.) Urb) is a local herb in the tropical region of Asia that has been selected to promote and develop to be one of the five best herbal products in Thailand due to its commercial competitiveness. Bua-bog has many properties, especially anti-bruising and anti-inflammatory properties. The objective of this study were to compare the anti-inflammatory effect of Bua-bog extract in the LPS-stimulated macrophages (RAW264.7) by using reverse transcription-polymerase chain reaction (RT-PCR) to study the expression of COX-2, IL-1β, IL-6, iNOS and TNF-α. After 24 hr incubation with macrophage cells, the crude extract of Bua-bog extracted with ethyl acetate (E1), absolute ethanol (E2), 50% ethanol (E3) and 80% ethanol (E4) found that E3 was the least toxic to cells, followed by E4, E2 and E1. In addition, various extracts of Bua-bog at concentrations of 50-200 µg/ml were able to significantly inhibit the expression of COX-2, IL-1β, IL-6, iNOS and TNF-α and close to indomethacin drugs. It was found that E1 and E2 showed better inhibition than those of E3 and E4. The results of this study are a report on the anti-inflammatory effect at the molecular level of Bua-bog extract, which is the information to support the development of natural products and pharmaceutical products from this plant.
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