Effect of Cytokinins and Explant Preparation on In Vitro Shoot Multiplication in Musa (AA group) ‘Khai Kamphaeng Phet’
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Abstract
In vitro propagation of Musa (AA group) ‘Khai Kamphaeng Phet’ encountered the problem with low shoot multiplication rate. This experiment aims to study the combination effect of cutting pattern and cytokinin on improving of shoot multiplication rate of Musa (AA group) ‘Khai Kamphaeng Phet’. 2 pieces-longitudinal cutting (2LC) of sterile shoot tips were cultured on solid Murashige and Skoog medium supplemented with 30 g/l sucrose and various types and concentration of cytokinins; Benzyladenine (BA), Kinetin (Kn) and Thidiazuron (TDZ) at 0, 0.5, 1.0, 2.0 and 4.0 mg/l for 6 weeks. An experimental design used in this study is Complete Randomized Design (CRD). The results found that the highest shoot induction number (2.6 shoots) was observed on the medium containing 0.1 mg/l TDZ. Hence, effects of TDZ concentrations and explant preparations on improving shoot multiplication efficiency were further examined. Different kind of explants; 2 pieces-longitudinal cutting (2LC) and 4 pieces-longitudinal cutting (4LC) segments, were cultured on solid MS medium augmented with TDZ at 0, 0.5, 1.0, 2.0 and 4.0 mg/l for 12 weeks. The results showed that the highest shoot induction number (5.2 shoots) could obtain when 2LC segments were cultured on the medium supplemented with 2.0 mg/l TDZ. In vitro shoots were transferred to culture on the medium supplemented with auxins; Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and Naphthaleneacetic acid (NAA) at 0, 0.5, 1.0, 2.0 and 4.0 mg/l for 6 weeks. The results revealed that the highest number of roots (19.9 roots) could obtain on the medium containing 2.0 mg/l IAA. Complete plantlets obtained from all the auxins-adding medium could survive at 100 % and grown vigorously after transplanting to greenhouse environment. This successful study could improve shoot multiplication rate in Musa (AA group) ‘Khai Kamphaeng Phet’ and acclimatized plantlets grew well with a healthy performance under greenhouse conditions.
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
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