Optimal Component Concentration of Preserving Agents for Control of Fungi Growth from Formalin-Fixed Human Cadavers
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Abstract
Faculty of Medicine, Princess of Naradhiwas University located in the south of Thailand with tropical climate. As a result of human cadavers that used for teaching has been contaminated by fungi. Even after the formalin preservation process. Later on at the end of every section class, the body were coated with preserving agents but they still contaminated with fungi. This study aims to identify the genus of fungi that grows on formalin-fixed human cadavers in the gross anatomy laboratory and to evaluate the optimal component concentration of preserving agents for control fungal growth. Seven fungal genera were identified in laboratory and on formalin-fixed human cadavers: Penicillium sp., Cladosporium sp.,Aspergillus sp., Aureobasidium sp., Chrysosporium sp., Sepedonium sp. and Alternaria sp.The Cladosporium sp. isolate B2, which most found on formalin-fixed human cadavers, was selected for the carbolic and glycerin in term of antifungal ability. The Central Composite Design (CCD) in composition of carbolic acid at 8% (v/v) and glycerin at 35%(v/v) showed the highest inhibition on Cladosporium sp. isolate B2 (39.43%). However carbolic acid at 8% concentration can cause toxicity for long term exposure. Therefore, the regression equation between carbolic acid and glycerin are important for predict the ability of fungi inhibition and minimize toxicity of carbolic acid.
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
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