Contents of Phenolic, Flavonoid and Antioxidant Activity of Dendrobium spp., in Maejo University, Chiang Mai Province
Main Article Content
Abstract
Flowers of Dendrobium orchids (Dendrobium lindleyi Steud., Dendrobium crumenatum Sw. and Dendrobium aphyllum (Roxb.) Fischer.) from Maejo University were investigated for total contents of phenolic and flavonoid compounds using folin-ciocalteu's reagent and aluminum chloride colorimetric methods. Their antioxidant activity was also performed using 2, 2-dyphenyl-1-picrylhydrazyl (DPPH) scavenging method. The results showed that Dendrobium lindleyi Steud. exhibited the highest total phenolic and flavonoid contents (39.81 mgGAE/g extract and 137.68 mgQE/g extract, respectively) and antioxidant activity
(IC50 = 0.21 mg/mL). There was a strong correlation (r=-0.925) between total phenolic compound content with antioxidant activity and intermediate correlation (r=-0.693) between total flavonoid content with antioxidant activity. The findings suggested that Dendrobium orchids may be a potential source of natural antioxidants especially with Dendrobium lindleyi Steud.
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
References
Cakova, V., Bonte, F. & Lobstein, A. (2017). Dendrobium: Sources of active ingredients to treat age related pathologies. Aging and Disease, 8(6), 827-849.
Das, S., Ray, A., Nasim, N., Nayak, S. & Mohanty, S. (2019). Effect of different extraction techniques on total phenolic and flavonoid contents, and antioxidant activity of betelvine and quantification of its phenolic constituents by validated HPTLC method. 3 Biotech, 9(37), 1-8.
Gupta. A. (2017). Antioxidants from medicinal plants and their relevance to human health. India: PP Savani University.
Halee, A. & Rattanapun, B. (2017). Study of antioxidant efficacies of 15 local herbsม KMUTT Research and Development Journal, 40(2), 283-293. (in Thai)
Jullapo, N. (2016). Phytochemical screening and biological activities of Dendrobium spp. Master’s Thesis. Burapha University. (in Thai)
Nachaikong, T., Wanyawa, A. Kaoian, K., Kongsooknirundorn, J., Supathirasakul, S., Wongnava, M. & Bumrungwong, N. (2014). Antioxidant and iron-chelating activity of some Thai medicinal plants, KKU Science Journal, 42(1),149-158.
Nguyen, H. A., Lin, K. H., Huang, M. Y., Yang, C. M., Shin, T. H., Hsiung, T. C., Lin, Y. C. & Tsao, F. C. (2018). Antioxidant activities of the methanol extracts of various parts of Phalaenopsis Orchids with white, yellow, and purple flowers, Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2),457-465.
Obsuwan. K., Jeong, B. R. & Maksup, S. (2019). Analysis of bioactive compounds, polysaccharides and antioxidant activity in different parts of Dendrobium Sonia Jo Daeng, Science, Engineering and Health Studies, 13(2), 73-82.
Phottraithip, A., Prapalert. W & Chotikadachanarong, K. (2019). Conservation and use of orchids in Ban Hua Thung Chiang Dao District Chiang Mai Province. Online. Retrieved November 27, 2020, from: http://www.cmruir.cmru.ac.th/bitstream/123456789/1929/5/Chapter2.pdf. (in Thai)
Phunsawan, B. (2013). Free radicals, Antioxidants and Antioxidant Activity Determination. Thai Journal of Science and Technology, 21(3), 275-286. (in Thai).
Prapalert. W, Phottraithip, W., & Chotikadachanarong, K. (2019). Study of the activity of phytochemicals in wild orchids. and propagation guidelines for communities. Online. Retrieved November 28, 2020, from: http://www.research.cmru.ac.th/research59/ris/view.php?no=904. (in Thai)
Prommajak, T., Surawang, S & Rattanapanone, N. (2014). Ultrasonic-assisted extraction of phenolic and antioxidative compounds from lizard tail (Houttuynia cordata Thunb ,Songklanakarin Journal of Science and Technology, 36(1), 65-72.
Rabeta, M. S. & Vithyia, M. (2013). Effect of different drying methods on the antioxidant properties of Vitex negundo Linn. Tea, International Food Research Journal, 20(6), 3171-3176.
Selamoglu, Z., Amin, K. & Ozgen, S. (2018). Plant secondary metabolites with antioxidant properties and human health. Turkey: Gece Publishing.
Shirazi, O. U., Khattak, M. M. A. K., Nor Shukri, A. M. & Nasyriq, M. N. A. (2014). Determination of total phenolic, flavonoid content and free radical scavenging activities of common herbs and spices, Journal of Pharmacognosy and Phytochemistry, 3 (3), 104-108.
Siriwattanamethanon, N. (2020). Multi-coloured food There are many benefits (Part 1): What are multicolored foods. Online Retrieved November 30, 2020, from:https://pharmacy.mahidol.ac.th/knowledge/files/0148.pdf. (in Thai)
Sridhar, K. & Charles, A. L. (2019). In vitro antioxidant activity of Kyoho grape extracts in DPPH and ABTS assays: Estimation methods for EC50 using advanced statistical programs, Food Chemistry,257,41-49.
Spiridon, I., Bodirlau, R. & Teaca, C. A. (2011). Total phenolic content and antioxidant activity of plants used in traditional Romanian herbal medicine, Central European Journal of Biology, 63(30), 388-396.
Tanagornmeatar, K., Sritularak, B. & Likhitwitayawuid. K. (2014). Chemical constituents of Dendrobium ellipsophyllum and their cytotoxic activity on KB cells. The 14th Graduate Researchconferences, February 22, 2014. Khon Koen: Khon Koen University. (in Thai)
Ueda, Y., Apiphuwasukcharoen, N., Tsutsumi, S., Matsuda, Y., Areekul, V. & Yasuda, S. 2019. Optimization of hot-water extraction of dried yacon herbal tea leaves: enhanced antioxidant activities and total phenolic content by response surface methodology, Food Science and Technology Research, 25(1), 131.139.