Efficiency of Dry Formulation Bacillus sp. Ks5 to Plant Growth Promotion and Biological Control of Xanthomonas oryzae pv. oryzae Caused Bacterial Leaf Blight Disease

Main Article Content

Suwichaya Buachard
Praphaphorn Phuangphee
Natthawan Jamsai

Abstract

Screening of antagonistic bacteria from rhizosphere soil of rice. Antagonistic bacteria were isolated of 23 isolates. 6 isolates showed the efficiency to inhibit of bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae. Bacillus sp. Ks5 indicad a difference higher inhibition of Xanthomonas oryzae pv. oryzae with clear zone 1.43 cm, using agar diffusion technique. Bacillus sp. Ks5 was adapted to the development of dry formulation and remarkably, this was successful as a carrier to maintain bacterial survival with 6.36×108 CFU/ml. The formulation from Bacillus sp. Ks5 was the most effective in inhibit bacteria pathogen that had been stored for 3 months with 2.20 cm, respectively. Dry formulation of Bacillus sp. Ks5 was able to produce plant growth promoters as ammonia.

Article Details

How to Cite
Buachard, S., Phuangphee, P., & Jamsai, N. (2022). Efficiency of Dry Formulation Bacillus sp. Ks5 to Plant Growth Promotion and Biological Control of Xanthomonas oryzae pv. oryzae Caused Bacterial Leaf Blight Disease. YRU Journal of Science and Technology, 7(2), 63–71. retrieved from https://li01.tci-thaijo.org/index.php/yru_jst/article/view/253810
Section
Research Article
Author Biographies

Suwichaya Buachard, Biology Program, Faculty of Science and Technology, Kamphaeng Phet Rajabhat University, KamphaengPhet 62000

Bacterial Leaf Blight Disease caused by Xanthomonas oryzae pv. oryzae has become a severe problem, mainly in Thailand. This research project aimed to test the efficacy of dry formulation for plant growth promotion and biological control of Xanthomonas oryzae pv. oryzae. Screening of antagonistic bacteria from the rhizosphere soil of rice. Antagonistic bacteria were isolated from 23 isolates. 6 isolates showed the efficiency to inhibit bacterial blight of rice caused by Xanthomonas oryzae pv.oryzae.
Bacillus sp. Ks5 indicated a difference in higher inhibition of Xanthomonas oryzae pv. oryzae with
clear zone 1.43 cm, using agar diffusion technique. Bacillus sp. Ks5 was adapted to the development of dry formulation and remarkably, this was successful as a carrier to maintain bacterial survival with 6.37×108 CFU/ml. The formulation from Bacillus sp. Ks5 was the most effective in inhibiting bacteria pathogens that had been stored for 3 months with 2.20 cm, respectively. The dry formulation of Bacillus sp. Ks5 was able to produce plant growth promoters such as ammonia.

Praphaphorn Phuangphee, Biology Program, Faculty of Science and Technology, Kamphaeng Phet Rajabhat University, KamphaengPhet 62000

Bacterial Leaf Blight Disease caused by Xanthomonas oryzae pv. oryzae has become a severe problem, mainly in Thailand. This research project aimed to test the efficacy of dry formulation for plant growth promotion and biological control of Xanthomonas oryzae pv. oryzae. Screening of antagonistic bacteria from the rhizosphere soil of rice. Antagonistic bacteria were isolated from 23 isolates. 6 isolates showed the efficiency to inhibit bacterial blight of rice caused by Xanthomonas oryzae pv.oryzae.
Bacillus sp. Ks5 indicated a difference in higher inhibition of Xanthomonas oryzae pv. oryzae with
clear zone 1.43 cm, using agar diffusion technique. Bacillus sp. Ks5 was adapted to the development of dry formulation and remarkably, this was successful as a carrier to maintain bacterial survival with 6.37×108 CFU/ml. The formulation from Bacillus sp. Ks5 was the most effective in inhibiting bacteria pathogens that had been stored for 3 months with 2.20 cm, respectively. The dry formulation of Bacillus sp. Ks5 was able to produce plant growth promoters such as ammonia.

Natthawan Jamsai, Biology Program, Faculty of Science and Technology, Kamphaeng Phet Rajabhat University, KamphaengPhet 62000

Bacterial Leaf Blight Disease caused by Xanthomonas oryzae pv. oryzae has become a severe problem, mainly in Thailand. This research project aimed to test the efficacy of dry formulation for plant growth promotion and biological control of Xanthomonas oryzae pv. oryzae. Screening of antagonistic bacteria from the rhizosphere soil of rice. Antagonistic bacteria were isolated from 23 isolates. 6 isolates showed the efficiency to inhibit bacterial blight of rice caused by Xanthomonas oryzae pv.oryzae.
Bacillus sp. Ks5 indicated a difference in higher inhibition of Xanthomonas oryzae pv. oryzae with
clear zone 1.43 cm, using agar diffusion technique. Bacillus sp. Ks5 was adapted to the development of dry formulation and remarkably, this was successful as a carrier to maintain bacterial survival with 6.37×108 CFU/ml. The formulation from Bacillus sp. Ks5 was the most effective in inhibiting bacteria pathogens that had been stored for 3 months with 2.20 cm, respectively. The dry formulation of Bacillus sp. Ks5 was able to produce plant growth promoters such as ammonia.

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