Efficiency of Dry Formulation Bacillus sp. Ks5 to Plant Growth Promotion and Biological Control of Xanthomonas oryzae pv. oryzae Caused Bacterial Leaf Blight Disease
Main Article Content
Abstract
Screening of antagonistic bacteria from rhizosphere soil of rice. Antagonistic bacteria were isolated of 23 isolates. 6 isolates showed the efficiency to inhibit of bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae. Bacillus sp. Ks5 indicad a difference higher inhibition of Xanthomonas oryzae pv. oryzae with clear zone 1.43 cm, using agar diffusion technique. Bacillus sp. Ks5 was adapted to the development of dry formulation and remarkably, this was successful as a carrier to maintain bacterial survival with 6.36×108 CFU/ml. The formulation from Bacillus sp. Ks5 was the most effective in inhibit bacteria pathogen that had been stored for 3 months with 2.20 cm, respectively. Dry formulation of Bacillus sp. Ks5 was able to produce plant growth promoters as ammonia.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
References
Azman, N.A., Sijam, K., Hata, E.M., Othman, R. & Saud, H.M. (2017). Screening of Bacteria as Antagonist against Xanthomonas oryzae pv. oryzae, the Causal Agent of Bacterial Leaf Blight of Paddy and as Plant Growth Promoter. Journal of Experimental Agriculture International, 16(4), 1-15.
Beric, T., Koji, M., Stankovi, S., Topisirovi, L., Degrassi, G., Myers, M., et al. (2012). Antimicrobial activity of Bacillus sp. natural isolates and their potential use in the bio¬control of phytopathogenic bacteria. Food Tech¬nology and Biotechnology, 50 (1), 25-31.
Chung, E.J., Hossain, M.T., Khan, A., Kim, K.H., Jeon, C.O. & Chung, Y.R. (2015). Bacillus oryzicola sp. nov., an Endophytic Bacterium Isolated from the Roots of Rice with Antimicrobial, Plant Growth Promoting, and Systemic Resistance Inducing Activities in Rice. The Plant Pathology Journal. 31(2), 152-164.
Dossa, C.S.G., Karlovsky, P. & Wydra, K. (2014). Biochemical Approach for Virulence Factors’ Identification in Xanthomonas Oryzae Pv. Oryzae. Journal of Plant Pathology & Microbiology, 5(2), 1-7.
Fan, B., Blom, J., Klenk, H.P. & Borriss, R. (2017). Bacillus amyloliquefaciens, Bacillus velezensis, and Bacillus siamensis Form an “Operational Group B. amyloliquefaciens” within the B. subtilis Species Complex. Frontiers in Microbiology, 8, 1-15.
Herrera, J.M., Rubio, G., Levy, L., Delgado, J.A., Lucho-Constantino, C.A., Islas-Valdez, S., et al. (2016). Emerging and established technologies to increase nitrogen use efficiency of cereals. Agronomy, 6(25), 1-19.
Jin, P., Wang, Y., Tan, Z., Liu, W. & Miao, W. (2020). Antibacterial activity and rice-induced resistance, mediated by C15surfactinA, in controlling rice disease caused by Xanthomonas oryzae pv. oryzae. Pesticide Biochemistry and Physiology. 169, 1-9.
Kantajan, A., Klinmanee, C. & Dhitikiattipong, R. (2018). Using Antagonistic Bacteria to Control Bacterial Blight of Rice under Greenhouse Condition. The 35th Winter Rice and Cereals Conference, June26 – 28, 2018. Chanthaburi: Sand Dunes Chaolao Beach Resort. (in Thai)
Kittiwongwattana, C., Thanaboripat, D., Laosinwattana, C., Koohakan, P., Parinthawong, N. & Thawa, T. (2016). Isolation of Plant-growth-promoting Bacteria from Rice (Oryza sativa). Journal of Science Ladkrabang, 25(1), 59–74. (in Thai)
Paslon, F.V., Alovera, R.B., Amper, C.D. & Ballentes, M.G. (2017). Suppression of Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) using Local Isolates of Rhizobacteria. CMU Journal of Science, 21, 2-8.
Poralokanon, P. (2017). Biochar based bioformula development of antagonistic bacteria to induce systemic resistant of organic lime against canker disease. Master’s Thesis. Thammasat University. (In Thai)
Public Health England. (2018). UK Standards for Microbiology Investigations: Identification of Bacillus species. London: Standards Unit.
Sirinunta, A. & Akarapisan, A. (2015). Screening of antagonistic bacteria for controlling Cercospora coffeicola in Arabica coffee. International Journal of Agricultural Technology, 11(5), 1209-1218.
Suliasih & Widawati S. (2020). Isolation of Indole Acetic Acid (IAA) producing Bacillus siamensis from peat and optimization of the culture conditions for maximum IAA production. The 9th International Symposium for Sustainable Humanosphere. October 28 – 29, 2019. Indonesia: Grand Savero Hotel.
Tariq, R., Ji, Z., Wang, C., Tang, Y., Zou, L., Sun, H., et al. (2019). RNA-Seq analysis of gene expression changes triggered by Xanthomonas oryzae pv. oryzae in a susceptible rice genotype. Rice, 12(44), 1-14.
Varma, p.K., Kumar, K.V.K., Sekhar, V.C., Adilakshmi, D., Suresh, M., Kumar, N.R., et al. (2017). Evaluation of Endophytic Bacteria for Plant Growth Promotion and Pathogen Suppression Traits in Saccharum officinarum. Journal of Agricultural Science and Technology A. 7, 535-545.
Wongchalee, D. (2015). Efficacy of an encapsulated antagonistic Bacillus in controlling chili anthracnose. Master’s Thesis. Suranaree University of Technology. (in Thai)
Wu, L., Wu, H., Chen, L., Yu, X., Borriss, R. & Gao, X. (2015). Difficidin and bacilysin from Bacillus amyloliquefaciens FZB42 have antibacterial activity against Xanthomonas oryzae rice pathogens. Scientific Reports, 5, 1 – 9.
Xie, S., Zang, H., Wu, H., Rajer, F.U. & Gao, X. (2018). Antibacterial effects of volatiles produced by Bacillus strain D13 against Xanthomonas oryzae pv. oryzae. Molecular Plant Pathology. 19(1), 49–58.
Yang, L., Quan, X., Xue, B., Goodwin, P.H., Lu, S., Wang, J., et al. (2015). Isolation and identification of Bacillus subtilis strain YB-05 and its antifungal substances showing antagonism against Gaeumannomyces graminis var. tritici. Biological Control, 8, 52-58.