Phytochemicals, Total Phenolic Compounds, and Antioxidant Activities of the Extracts of Shoots and Stems of Bua-Bok-Khod (Stephania pierrei)
Article Sidebar
Main Article Content
Abstract
Antioxidants can play a role in destroying free radicals which cause disease and illness. Antioxidants can be found in fruits and vegetables, especially medicinal plants. The objective of this research was to screen the phytochemicals and analyze the total phenolic compounds (TPC), total flavonoids (TF), and antioxidant activities of the extracts of shoots and stems of (Stephania pierrei), which were extracted by water, ethanol, and chloroform. The results showed that alkaloids, flavonoids, saponins and tannins were found in both parts of S. pierrei. The shoot extracts exhibited higher TPC than the stem extracts. In contrast, the shoot extracts showed lower TF than the stem extracts. The highest levels of TPC and TF were obtained from the ethanol extract of stems and the chloroform extract of shoots, which corresponding to 210.52 mg GAE/g extract and 44.78 mg QE/g extract, respectively. Antioxidant activities of the extracts were analyzed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH assay) and ferric reducing antioxidant power (FRAP assay). In both assays, the stem extracts showed higher antioxidant activities than shoot extracts. Moreover, the ethanol extract of stems also demonstrated the highest level of antioxidant activities, with the SC50 and FRAP values of 0.186 mg/ml and 1.622 mmol Fe(II)/g extract, respectively. The results indicated that the extracts of S. pierrei shoots and stems had antioxidant properties. Moreover, optimization of extracting process and analysis of the substances that have antioxidant activities should be studied for further development of their into health products.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
References
Al-Amin, M. Y., Lahiry, A., Ferdous, R., Hasan, M. K., Kader, M. A., Alam, A. H. M. K. et al. (2022). Stephania japonica ameliorates scopolamine-induced memory impairment in mice through inhibition of acetylcholinesterase and oxidative stress. Advances in Pharmacological and Pharmaceutical Sciences, 8305271, 1-17.
Benhawan, A. & Waema, S. (2021). Identification of phenolic compounds in Melastoma malabathricum (L.) extract by HPTLC technique and their food pathogenic bacteria activities. Journal of Science and Technology, 6(1), 81-90. (in Thai).
Bor, J. Y., Chen, H. Y. & Yen, G. C. (2006). Evaluation of antioxidant activity and inhibitory effect on nitric oxide production of some common vegetables. Journal of Agricultural and Food Chemistry, 54(5), 1680-6.
Buachoon, N. (2020). Phytochemical screening and antioxidant activity of fresh and dried nasturtium officinal plants. EAU Heritage Journal Science and Technology, 14(1), 144-154. (in Thai).
Chansri, R. & Kongkum, N. (2022). Activities of culinary herbs for antioxidants, antimicrobial and anti-inflammatory. Journal of Science and Technology Mahasarakham University, 42(5), 145-154. (in Thai).
Dary, C., Hul, S., Kim, S. & Jabbour, F. (2015). Lectotypification of Stephania pierrei (Manispermaceae) Edinb. Journal of Botany, 72(3), 423-428.
De, S., Dey, Y. & Ghosh, A. (2010). Phytochemical investigation and chromatographic evaluation of the different extracts of tuber of Amorphaphallus paeoniifolius (Araceae). International Journal on Pharmaceutical and Biological Research, 1(5), 150-157.
Denchai, R., Phaiboon, N., Pornsiri, N. & Rattana, S. (2021). Antioxidant activities, total phenolic and total flavonoid contents of Lepisanthes rubiginosa crude extract. Koch Cha Sarn Journal of Science, 43(2), 1-9. (in Thai).
Dordevic, T. M., Marinkovic, S. S. S. & Brankovic, S. I. D. (2010). Effect of fermentation on antioxidant properties of some cereals and pseudo cereals. Food Chemistry, 119(3), 957-963.
Kamonwannasit, S. & Kamcharoen, A. (2018). Phytochemical screening and antibacterial activities of tuber extract of Stephania pierrei Diels. Chiang Mai Journal of Science, 45(3), 1396-1406.
Kamonwannasit, S., Prajanban, B., Srimee, P. & Kamcharoen, A. (2022). Anti-inflammatory and anti-oxidative activities of Stephania pierrei extracts. HCU Journal, 26(1), 25-34.
Kamonwannasit, S., Rupitak, Q. & Kamcharoen, A. (2019). Antifungal and antioxidant activities of the extract of Stephania pierrei tubers. The 4ed RSU International Research Conference, April 26, 2019. Pathum Thani: Rangsit University.
Khongpet, W., Sanghkawisu, P., Makerd, W. & Namjan, M. (2023). Phytochemical screening, total phenolic contents and antioxidant activity of Curcuma putii maknoi & jenjitt. extracts. Life Sciences and Environment Journal, 24(1), 14-25. (in Thai)
Likhitwitayawuid, K., Angerhofer, C. K., Chai, H., Pezzuto, J. M., Cordell, G. A., Ruangrungsi, N. (1993). Cytotoxic and antimalarial alkaloids from the tubers of Stephania pierrei. Journal of Natural Products, 56(9), 1468-78.
Munteanu, I. G. & Apetrei, C. (2021). Analytical methods used in determining antioxidant activity: A review. International Journal of Molecular Sciences, 22, 3380.
Painupong, A. (2017). Free radicals and anti-oxidants in human health. PKRU SciTech Journal, 1(2), 20-27. (in Thai).
Prior, R. L., Wu, X. & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. The Journal of Agricultural and Food Chemistry, 53, 4290-4302.
Sembiring, E. N., Elya, B. & Sauriasari, R. (2018). Phytochemical screening, total flavonoid and total phenolic content and antioxidant activity of different parts of Caesalpinia bonduc (L.) Roxb. Pharmacognosy Journal, 10(1), 123-127.
Singsai, K., Sakdavirote, A., Wechpanishkitkul, K. & Moonsamai, A. (2020). The comparison of phenolic compounds, flavonoids and antioxidant activities of the ethanolic extracts of shoots, leaves, fruits and seeds of Leucaena leucocephala. Naresuan Phayao Journal, 13(3), 66-73. (in Thai).
Sonsupab, B. (2015). Study and survey of genetic resources and the distribution of herbs protection for the purpose of plant varieties protection and conservation. (Research report). Department of Agriculture, DOA: Plant Varieties Protection Office. (in Thai).
Sonsupab, B. (2019). Research on species diversity and identification and utilization of indigenous plants in according to Plant Variety Protection Act B.E. 2542. (Research report). Department of Agriculture, DOA: Plant Varieties Protection Office. (in Thai).
Yadav, R. & Agarwala, M. (2011). Phytochemical analysis of some medicinal plants. Journal of Phytology, 3(12), 10-14.