Optimization of protoplast fusion conditions of vetiver grass (Vetiveria zizanioides)
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Abstract
Vetiver (Vetiveria zizanioides) belongs to Poaceae family which is used for soil and water conservation. The improvement of this grass through the conventional breeding is very limited due to male sterility. Protoplast fusion technology is a useful technique that can be solved this limitation in vetiver improvement. Therefore, this study aimed to optimize the protoplast fusion conditions for somatic hybridization between two vetiver grass varieties, including Kamphaeng Phet 2 (Vetiveria zizanioides Nash) and Prachuap Khiri Khan (Vetiveria nemoralis A. Camus) by polyethylene glycol (PEG)-mediated method. The mesophyll protoplasts of both varieties were incubated with various combination between concentrations of PEG 6000 (10, 20 or 30%) and incubation periods (10, 20 or 30 min). The experimental design is 4×3 completely randomized factorial design (4×3 factorial in CRD) with 3 replications. The results showed that the highest percentage of binary fusion (43.45%) and the viability of protoplasts (89.63%) were obtained from 10% PEG 6000 with 30 min incubation period. This research is the preliminary study which is useful to produce somatic hybridization for vetiver improvement in the future.
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
References
Badr-Elden, A. M., Nower, A. A., Nasr, M. I. & Ibrahim, A. I. (2010). Isolation and Fusion of Protoplasts in Sugar Beet (Beta vulgaris L.). Sugar Tech, 12(1), 53-58.
Chakraborty, S. (2016). Protoplast Culture and Somatic Hybridization, A Promising Frontier of Plant Biotechnology. A Peer Reviewed Multidisciplinary Research Journal, 2(1), 46-54.
Guan, Q., Guo, Y., Wei, Y., Meng, F. & Zhang, Z. (2010). Regeneration of Somatic Hybrids of Ginger via Chemical Protoplast Fusion. Plant Cell, Tissue and Organ Culture, 102, 279-284.
Gu, J. & Catchmark, J. M. (2013). The Impact of Cellulose Structure on Binding Interactions with Hemicellulose and Pectin. Springer Science and Business Media, 20, 1613-1617.
Hayeehateng, A. & Kaewnok, B. (2014). Campaign to Encourage of Vetiver Grass Cultivation for Soil and Water Conservation Under the Royal Initiative of His Majesty the King, Yala Province. Phatthalung: Land Development Department. Ministry of Agriculture and Cooperatives. (in Thai)
Kao, K. N., Constabel, F., Michayluk, M. R. & Gamborg, O. L. (1974). Protoplast Fusion and Growth of Intergeneric Hybrid Cells. Plant Biology, 115, 215-227.
Kativat, C. (2014). Protoplast Culture and Development of Sunflower Maintainer Line via Protoplast Fusion. Master’s Thesis. Suranaree University of Technology. (in Thai)
Kativat, C. & Tantasawat, P. A. (2017). PEG-mediated Symmetric and Asymmetric Protoplast Fusion in Sunflower (Helianthus Annuus L.). Pakistan Journal of Botany, 49(5), 1903-1910.
Keegstra, K. (2013). Plant Cell Walls. Plant Physiology Journal, 154, 483–486.
Mansour, M. M. F. (2013). Plasma Membrane Permeability as An Indicator of Salt Tolerance in Plants. Biologla Plantarum, 57(1), 1-10.
Murashige, T. & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Plant Physiology Journal, 15, 473-497.
Parihar, A., Pachchigar, K. & Zaman, M. S. (2015). Protoplast Isolation and Fusion in Cultivated Cajanus cajan (L.) Millsp. and Wild Species of Pigeonpea. Research Journal of Biotechnology, 10(3), 62-72.
Shalsh, F. J., Ibrahim, N. A., Arifullah, M. & Hussin, A. S. M. (2016). Optimization of the Protoplast Fusion Conditions of Saccharomyces cerevisiae and Pichia stipitis for Improvement of Bioethanol Production from Biomass. Asian Journal of Biological Sciences, 9(1-2), 10-18.
Sivaarthikul, K. (2015). Sustainable Management of Vetiver Utilization. Bangkok: Land Development Department, Ministry of Agriculture and Cooperatives. (in Thai)
Tomar, U.K. & Dantu, P.K. (2010). Protoplast Culture and Somatic Hybridization. In Tripathi, G. Cellular and Biochemical Sciences. I.K. International Publishing House Pvt. Ltd., New Delhi, 876-891.
Tudses, N., Premjet, S. & Premjet, D. (2014). Optimal Conditions for High-Yield Protoplast Isolations of Jatropha curcas L. and Ricinus communis L. American-Eurasian Journal of Agricultural and Environmental Science, 14(3), 221-230
Tudses, N., Premjet, S. & Premjet, D. (2015). Establishment of Method for Protoplast Fusion with PEG- Mediated between Jatropha curcas L. and Ricinus communis L. International Journal of Life Sciences Biotechnology and Pharma Research, 4(1), 50-56.
Wulandari, D. R., Purwito, A., Susanto, S., Husni, A. & Ermayanti, T. M. (2018). Protoplast Fusion between Indonesian Citrus maxima (Burm.) Merr. and Citrus reticulata L.: A Preliminary Report. Agrivita Journal of Agricultural Science, 40(2), 233-241.
