Validation of Molecular Markers Used for Assisting Selection of Cassava (Manihot esculenta Crantz) with Low Starch Pasting Temperature
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Abstract
Marker assisted selection (MAS) technology used for assisting selection of starch pasting viscosity traits is an important goal for breeding of cassava (Manihot esculenta Crantz) to support the tapioca starch industry. This research aimed to evaluate the molecular markers linked to starch pasting viscosity in F1 population derived from 13 crosses. The genotyping results showed that the band pattern of 54 F1 lines revealed 4, 7, 8 and 2 alleles for MeES959, MeES1019, SSRY13 and EME81 markers, respectively. Starch pasting viscosity values showed statistically significant difference (p<0.01) among F1 lines. The average phenotypic values of PV, HS, FV, PT, PTi, BD and SB were 200.9 cP, 56.0 cP, 104.6 cP, 68.6oC, 210.6 seconds, 145.0 cP and 48.6 cP, respectively. The marker-trait efficiency was validated in F1 population using simple linear regression and ANOVA test. It was found the relationship between genotypic class and starch pasting viscosity. Among four markers, EME81 was the most effective marker for selecting the F1 lines consisted of low- and high-pasting viscosity. The allele 1 contributed to the lower PT. The PT was classified into 3 classes depending on the genotypic class (1/1, 1/2 and 2/2).
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
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