Effects of fertilizer type on protein and carbohydrate fractions of two varieties of Arachis hypogaea fodder
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Abstract
The fractionation of protein and carbohydrate of two varieties of A. hypogaea affected by fertilizer type was investigated. The crude protein (CP) of the A. hypogaea varieties was subdivided into five fractions; A1 (crude protein soluble in the borate-phosphate buffer and tungstic acid solution), B1 (true protein soluble in buffer solution and precipitated by the tungstic solution), B2 (true protein insoluble in buffer solution but soluble in the neutral-detergent solution), B3 (true protein soluble in acid-detergent solution but insoluble in neutral-detergent solution), and C (true protein insoluble in the acid-detergent
solution). While the carbohydrate (CHO) was fractionalized into four; CA (rapidly degradable CHO), CB1 (intermediately degradable CHO), CB2 (slowly degradable CHO), and CC (completely undegradable NDF). The experiment was a 3 × 2 factorial in split-plot design, with the fertilizer type as the main plot (control, NPK, and poultry droppings) and the variety as the sub-plot (SAMNUT 22 and Local). The total treatment combination is six with three replications. The fertilizer type affected all the CP fractions except for fraction C. The interaction of fertilizer type × variety influenced all the CP fractions with a range of 65.40 ± 0.21 to 80.97 ± 0.01 g/kg DM, 5.01 ± 0.01 to 5.69 ± 0.03 g/kg DM, 42.77 ± 0.08 to 51.40 ± 0.01 g/kg DM, and 58.34 ± 0.14 to 69.93 ± 0.01 g/kg DM for CP fraction A, B1, B2, and B3, respectively with the organic fertilized local variety having the highest values. SAMNUT 22 without fertilizer recorded the highest value for CP fraction C. Varietal difference was observed for all the CP fractions. The CHO fractions were affected (P < 0.05) by the fertilizer type and varietal difference. The unfertilized SAMNUT 22 was observed to have the highest values for CHO fraction CA, CB1, and CC, whereas the same variety to which inorganic fertilizer was applied recorded the highest value for CB2. The organic fertilized local variety of A. hypogaea had a higher portion of the soluble fraction of CP, and a lower portion of cell wall bounded CP fraction making it ruminally beneficial if fed to ruminants which also reflected in the utilizable CP.
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