การวิเคราะห์องค์ประกอบทางเคมีในยอดอ้อยแบบรวดเร็วด้วยเนียร์อินฟราเรด สเปกโตรสโคปี

Abstract

Sugarcane tops are crop residues and one of a major by-product of sugarcane production. They have been used as an alternative ruminant forage and a material for bioethanol synthesis. Chemical compositions inside them have a strong influence on the ethanol yield and are necessary for balancing feed ration. The objective of this study was to evaluate the possibility of near infrared (NIR) spectroscopy for a rapid determination of chemical compositions in sugarcane tops. Fresh sugarcane tops were harvested and evaluated for contents of dry matter, crude protein, ash, NDF, ADF, cellulose and lignin. The sugarcane tops were chopped and used for the NIR measurements which were performed using reflectance FT-NIR spectroscopy in the region of 9000-4000 cm^-1. Data analysis was performed using partial least square (PLS) regression. The PLS models developed for all chemical contents had satisfied correlation coefficient of determination (R) values greater than 0.91. The values of root mean square error of cross validation (RMSECV) from the models of dry matter, crude protein, ash, NDF, ADF, cellulose and lignin were 1.44, 0.27, 0.48, 4.14, 1.75, 0.39 and 0.38%, respectively. The results suggested that NIR spectroscopy had a potential to be a rapid and effective method for the determination of chemical composition in sugarcane tops. This method could be a powerful tool for quality evaluation of agricultural by-product.