In vitro Galangal (Alpinia galanga (L.) Willd) to Produce Bacterial Wilt Disease Free Plants using tissue culture technique

Abstract

The objective of this study was to investigate the effect of plant growth regulators on shoot multiplication and root induction in Galangal at the Phichit Agricultural Research and Development Center. The shoot multiplication experiment was conducted using a completely randomized design (CRD) with six treatments and three replications. Young shoot bud explants were cultured on MS medium supplemented with BA concentrations at 0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/L. The result showed that MS medium containing 5.0 mg/L BA produced the highest number of shoot, averaging 3.83 shoots per explant. Additionally, it yielded the highest shoot height and leaf number, with mean values of 6.22 cm and 2.81 leaves, respectively, after 12 weeks of culture. For root induction experiment, a CRD with seven treatments and three replications. Shoot explants were cultured on MS medium supplemented with 5.0 mg/L BA in combination NAA or IAA concentrations of 0.0, 0.5, 1.0 and 2.0 mg/L. The result revealed that MS medium supplemented with 5.0 mg/L BA and 2.0 mg/L IAA promoted the highest root number, averaging 6.50 roots per explant, as well as the highest shoot number, shoot height and leaf number, with value of 4.25 shoots, 8.24 cm and 5.77 leaves, respectively, after 12 weeks of culture. When cultured for 16 weeks, this treatment resulted in the highest shoot number at 5.19 shoots, root number at 16.9 roots and leaf number at 6.23 leaves, respectively. Overall, young Galangal plants exhibited optimal growth before being transplanted to the nursery.