Genetic Charaterization of Isolates of Fusarium oxysporum f. sp. lycopersici as Determined by ARDRA and RAPD Analysis

บทคัดย่อ

Twelve isolates of Fusarium oxysporum f. sp. lycopersici from Thailand, the causal organism of Fusariumwilt of tomato, were examined for ITS1-5.8S-ITS2 region by amplified ribosomal DNA restriction analysis (ARDRA)and genomic DNA by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). All isolatescould be distinguished from each other by ARDRA analysis, and clustered into four groups (A, B, C and D) usingunweighted pair group method with arithmetic average (UPGMA) analysis. The isolates were grouped into twogroups (E and F) by UPGMA analysis based on RAPD-PCR data. There was similarity between the UPGMAdendrograms of ARDRA and RAPD-PCR analysis, all of the pathogenic isolates were in group A and E,respectively. Pathogenic isolates have been clearly distinguished from non-pathogenic ones by RAPD-PCR. Thisstudy showed that, at the DNA level pathogenic isolates are more closely related, whereas non-pathogenic isolatesare more diverse.