Enhancement of L-Ergothioneine in Khon (Lentinus spp.) and Inkcap (Coprinus comatus) Mushrooms by fortifying substrate with L-histidine

Abstract

Growth characteristics and fruiting on histidine (His) amended PDA and levels of L-ergothioneine (ERG) in fruiting bodies of various mushroom species were determined. It was found that growth and mycelial density of some mushroom species were interfered with the higher concentrations of His and the ability to produce fruiting bodies of some species was impaired. The addition of 500 mg/L in PDA produced slender mycelial density of khon mushrooms species such as Lentinus conatus and Lentinus villosus. The levels of ERG accumulated in fruiting bodies depended on mushroom species. The addition of ≤100 mg/L stimulated inkcap mushroom (Coprinus comatus) to accumulate ERG but did not in khon mushrooms (Lentinus polychrous and Lentinus squarrosulus). Taking these results into consideration, suggested that the addition of His into substrate is an effective mean to enhance the accumulation of antioxidant properties in inkcap mushroom but needs more study on khon mushrooms.