Evaluation of chili pepper progressive lines (Capsicum annuum L.) derived anthracnose resistant genes from PBC80 and PBC932 varieties resistance to Colletotrichum acutatum
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Abstract
Chili anthracnose disease caused chili fruit rot both preharvest and postharvest. Therefore, this study aimed to evaluate resistance of chili pepper to anthracnose disease in the 21 chili progressive lines derived from PBC80 and PBC932 compared with commercial variety and susceptible check. Three chili fruit stages (green breaking and ripe) were inculcated C. accutatum (Ca_KK) isolate with a spore suspension of anthracnose to chili plant at 75 days after transplant by sprayed on hold plant inoculation. The experiments were designed by randomized complete block design (RCBD) with three replications and three plants per each. The disease was evaluated at 14 days after inoculation, and the disease was calculated to a percentage of disease index (DI%). For the genotypic data, two DNA markers SSR-HpmsE032 and SCAR-Indel, were used. All chili genotypes showed significantly different responses to Ca_KK in three fruit stages (green breaking and ripe). Five progressive lines (ANT4, ANT6, ANT9, ANT17, and ANT18) showed resistance in the green fruit stage, two lines (ANT 4 ANT 12) were resistant in the ripe fruit stage, while all lines showed susceptibility in the breaking fruit stage. The correlation of resistance to anthracnose among three different fruit mutuality stages, i.e., green, breaking, and ripe fruit stages were found ranging from 0.41-0.56%. For the genotypic evaluation, SSR-HpmE032 detected the resistant gene in two chilli progressive lines (ANT 4 ANT 17), while SCAR-Indel was detected in three progressive lines (ANT1 ANT9 and ANT10). Both markers were associated for selection with C. accutatum (Ca_KK) ranging percentage of 71.42-90.47.
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