Development of a bacterial growth tracker (BGT), a smart device to detect growth of Vibrio in shrimp farm
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Abstract
Bacteria is major microorganism in the shrimp pond, which can be both beneficial and pathogenic bacteria. The smart device called “bacterial growth tracker (BGT)” to detect growth of bacteria in shrimp farm that can be performed by farmers, resulting to apply the pond management in a timely manner has been developed. The objective of this study was to develop a detection method of Vibrio species in shrimp ponds. The modified liquid medium for Vibrio (MTCBS) has been developed and tested to be able to grow only Vibrio species tested. The selective MTCBS medium was then combined with BGT to detect growth of Vibrio spp. BGT is composed of two major parts: (1) the incubator shaker with a compartment for a medium tube and with a temperature control set at 30oC (2) the light transmittance sensor to measure turbidity of medium culture caused by bacterial growth and record the light transmittance in real time at every five minutes of interval. The selective MTCBS medium for Vibrio has been used in BGT device to determine Vibrio counts in the shrimp culture water. The Vibrio bacterial growth test was compared between the BGT and the spectrophotometer in laboratory. This study has successfully developed a prototype BGT that can be further used in the farm.
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