The effect of non-thermal plasma on DNA methylation-related gene expression in crossbred Pradu Hang Dam chicks

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Published: Sep 28, 2023
Keywords:
gene expression crossbred Thai native chicken DNA methylation cold plasma

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Siripatsorn Thinpa
Division of Animal Science and Feed Technology, Department of Agricultural Sciences, Faculty of Agriculture Natural Resources and Environment, NaresuanUniversity, Phitsanulok
Worasak Kaewkong
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok
Pakpoom Subsoontorn
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok
Choncharoen Sawangrat
Department of Physica and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai
Dheerawan Boonyawan
Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai
Tiranun Srikanchai
Division of Farm technology management, Faculty of Agroindustry, Panyapiwat Institute of Managements, Nontaburi
Apichaya Sakulthai
Division of Farm technology management, Faculty of Agroindustry, Panyapiwat Institute of Managements, Nontaburi
Pairat Srichana
Charoen Pokphand Foods Public Company Limited (CPF), Bangkok
Thitima Pechrkong
Division of Animal Science and Feed Technology, Department of Agricultural Sciences, Faculty of Agriculture Natural Resources and Environment, NaresuanUniversity, Phitsanulok
Rangsun Charoensook
Division of Animal Science and Feed Technology, Department of Agricultural Sciences, Faculty of Agriculture Natural Resources and Environment, NaresuanUniversity, Phitsanulok

Abstract

The aim of this study was to investigate the effect of Cold plasma on DNA methylation-related gene expression in crossbred Thai native chickens. Fourth day-incubation fertilized eggs, which is the optimal stage for the beginning of embryo development were exposed with cold plasma. Incubated eggs were exposed for 10, 20 and 30 seconds, respectively, compared to the control group. Expression of DNA methylation-related genes (DNMT1, DNMT3a and DNMT3b) was determined in liver tissues derived from 32 a day-old chick (n=8 per group) by real-time PCR techniques. Significant downregulation of DNMT3b gene was found in all plasma-treat groups (P=0.0014, P<0.0001, P=0.003, respectively). This study demonstrated that cold plasma technology had affected on DNA methylation-related gene expression, which affects the development of the embryo and the regulation of the expression of other genes related to development of the chicken embryo. This green technology will be saving as the further development strategy for agricultural benefits in Thailand.

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How to Cite
Thinpa, S. ., Kaewkong, W. ., Subsoontorn, P. ., Sawangrat, C. ., Boonyawan, D. ., Srikanchai, T. ., Sakulthai, A., Srichana, P. ., Pechrkong, T. ., & Charoensook, R. . (2023). The effect of non-thermal plasma on DNA methylation-related gene expression in crossbred Pradu Hang Dam chicks . Khon Kaen Agriculture Journal, 51(5), 856–866. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/256167
Issue
Vol. 51 No. 5 (2566): (September-October)
Section
บทความวิจัย (research article)
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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