Isolation and characterization of plant-growth-promoting rhizobacteria from the rhizospheric soil of selected pulses and their effect on Allium fistulosum L. and Capsicum frutescens L.

Main Article Content

Achiraya Siriphap
Wanwisa Pirapak
Parinya Kraivuttinun

Abstract

Plant-growth-promoting bacteria (PGPB) have various direct and indirect mechanisms of action. In this study, six optimal plant-growth-promoting rhizosphere bacteria (L9-05, L45-05, L10-04, L2-03, L2-04, and L41-01) were isolated from the root zone of healthy, vigorously growing plants from a farm in Uttaradit province, Thailand. All the selected bacterial isolates promoted the growth of Allium fistulosum L. and Capsicum frutescens L. Moreover, all the isolates were selected based on their ability for nitrogen fixation, indole-3-acetic acid (IAA) production, and phosphate solubilization. Based on the morphological traits and 16S rRNA sequence analysis, we found that bacterial isolate L10-04a strain of Enterobacter hormaechei promoted the growth and increased the yields of Capsicum frutescens L. Thus, this strain is suitable for application in organic farming.

Article Details

How to Cite
Siriphap, A. ., Pirapak, W. ., & Kraivuttinun, P. (2025). Isolation and characterization of plant-growth-promoting rhizobacteria from the rhizospheric soil of selected pulses and their effect on Allium fistulosum L. and Capsicum frutescens L. Khon Kaen Agriculture Journal, 53(2), 302–311. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/262192
Section
บทความวิจัย (research article)

References

จตุพร บุณณดากุล, ศิราภรณ์ ชื่นบาล, ฐปน ชื่นบาล, ศรีกาญจนา คล้ายเรือง และศุภธิดา อํ่าทอง. 2562. การคัดเลือกแบคทีเรียตรึง ไนโตรเจนอิสระเพื่อส่งเสริมการเจริญเติบโตของข้าว. Naresuan Phayao Journal. 12: 32–40.

AOAC. 2000. Official methods of analysis. Association of Official Analytical Chemists, Washington. 17th ed.

Andy, A. K., S. A. Masih, and V. S. Gour. 2020. Isolation, screening and characterization of plant growth promoting rhizobacteria from rhizospheric soils of selected pulses. Biocatalysis and Agricultural Biotechnology. 27: article ID 101685.

Chen, J., G. Zhao, Y. Wei, Y. Dong, L. Hou, and R. Jiao. 2021. Isolation and screening of multifunctional phosphate solubilizing bacteria and its growth-promoting effect on Chinese fir seedlings. Scientific Reports. 11: article ID 9081.

Edith, M., and M. T. Garcia. 2007. Plant growth stimulation by Burkholderia cepacia, a native rhizobacteria of acid soils in Venezuelan savannas. Agronomía Tropical. 57: 123-128.

Goswami, M., and S. Deka. 2020. Plant growth-promoting rhizobacteria alleviators of abiotic stresses in soil: A review. Pedosphere. 30: 40-61.

Kizhakedathil, J., and C. Devi. 2018. Rhizospheric bacteria isolated from the agricultural fields of Kolathur, Tamilnadu promotes plant growth in mustard plants. Biocatalysis and Agricultural Biotechnology. 16: 293-302.

Latt, Z. K., S. Y. San, E. P. Kyaw, T. M. Lynn, M. T. Nwe, W. W. Mon, and K. N. Aye. 2018. Using cellulolytic nitrogen-fixing bacterium, Azomonas agilis for effective degradation of agricultural residues. Open Microbiology Journal. 12: article ID 154.

Nathiya, S., J. Rajendran, and R. K. Velu. 2020. Potential of plant growth promoting rhizobacteria to overcome the exposure to pesticide in Trigonella foenum-graecum (fenugreek leaves). Biocatalysis and Agricultural Biotechnology. 23: article ID 101493.

Pirapak, W., A. Siriphap, T. Inprasit, C. Phuangsri, and P. Kraivuttinun. 2022. Isolation and characterization of plant growth promoting rhizobacteria from rhizospheric soil of selected pulses and their effect on Coriandrum sativum plants. Biodiversitas. 23: 5765-5770.

Pradhan, S., and M. R. Pokhrel. 2013. Spectrophotometric determination of phosphate in sugarcane juice, fertilizer, detergent, and water samples by molybdenum blue method. Scientific World Journal. 11: 58-62.

Sage, R. F., R. W. Pearcy, and J. R. Seemann. 1987. The nitrogen use efficiency of C3 and C4 plants. Plant Physiology. 85: 355-359.