Effect of Caesalpinia sappan L. extract and Silver ions on inhibition mastitis-causing bacteria pathogens in dairy cows
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Abstract
Most cases of mastitis in dairy cows are caused by bacterial infections. Irrational use of antibiotics leads to antibiotic residue and increases the incidence of drug resistance of bacteria. The use of herbs or other substances is therefore an alternative to the use of antibiotics. Caesalpinia sappan L. is an herb that has the effect of inhibiting bacteria. Additionally, silver ions have the effect of killing bacteria, viruses, and fungi. The objective of this study was to evaluate the efficacy of Caesalpinia sappan L. extracts and silver ions to inhibit mastitis causing bacteria pathogens in dairy cows. The minimum inhibitory concentration method (MIC), uses Caesalpinia sappan L. and silver ions, was tested for inhibition of mastitis-causing bacteria pathogens in dairy cows. The concentrations of Caesalpinia sappan L. extract evaluated were 20, 10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 0.7, 0.04, 0.02 and 0.02 mg/ml, and the concentration of the silver ion evaluated were 2, 1, and 0.5 mM/mL. The MIC value of Caesalpinia sappan L. was between 0.04 and 2.5 mg/mL. The lowest MIC value of Caesalpinia sappan L. extract was 0.04 mg/mL for Enterococcus faecalis. The highest MIC value of Caesalpinia sappan L. extract was 2.5 mg/mL for Streptococcus uberis. In addition, The MIC value of silver ions was 0.5 mM/mL for all mastitis-causing bacteria. It was concluded that Caesalpinia sappan L. and silver ions can be applied in commercial production processes.
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References
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