Phytochemical contents and antioxidant activities of ethanolic and aqueous extracts from Indigofera suffruticosa Mill

Abstract

            The objective of this study was evaluated the phytochemical contents and the antioxidant activity of Indigofera suffruticosa Mill. extracted with 95%, 50% ethanol and aqueous. The chemical contents of the indugo leaves studying by measuring the amount of total phenolic content, total flavonoid content and chlorophyll content. For antioxidant activity, DPPH assay, ABTS assay and FRAP assay were studied. From this study found that indigo leaves extracts with 95% ethanol showed the highest amount of total phenolic contents and chlorophyll (30.37 ± 0.09 µg GAE/g DW and 0.14 ± 0.00 mg/g, respectively), when compared with 50% ethanol and aqueous. For the study of antioxidant activity by DPPH assay, ABTS assay and FRAP assay, it was found that indigo leaves extracts with 95% ethanol had better antioxidant activity than indigo leaves extracts with 50% ethanol and aqueous, by DPPH assay and ABTS assay, the IC₅₀ value were 26.14 ± 0.29 µg/mL and 19.88 ± 0.93 µg/mL, respectively and the FRAP assay had an antioxidant value of 239.73 ± 0.17 mg FeSO₄ equivalent/g extract. The relationship of between phytochemical contents and antioxidant activities, it was found that the phenolic contents had the most relationship with the antioxidant activity.