Collection and Classification of Commercial Strain of Shiitake Mushroom by Molecular Marker; RAPD
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Abstract
Fourty-three commercial strains of shiitake mushroom, Lentinus edodes (Berk.) Sing. collected from northern area in Chiang Mai, Chiang Rai, Lampang and Lamphun, were evaluated the genetic diversity and strain identification by random amplified polymorphic DNA (RAPD) technique. Genomic DNA was extracted from fresh mushroom and RAPD analysis was performed using 27 decamer primers. 21 out of 27 primers produced 99 polymorphic bands. A total of 178 amplification products were scored of which 55.62% were polymorphic. Ten primers gave identical polymorphic band in a particular strain which could be used to distinguish that strain from the others. The identical bands were observed in four strains ‘Royal project’, ‘Mit’, ‘Wilai’ and ‘Anuchit’. The genetic similarity index of these strains ranged from 0.311 to 0.963. A dendrogram generated by Unweighted Pair Group Method (UPGMA) analysis showed that the commercial strain of shiitake clustered into 6 groups. Molecular genetic markers obtained with the RAPD technique can be used to differentiate commercial strains of L. edodes.
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