Chromosome Investigation of <I>Liparis siamensis </I> Rolfe ex Downie and <I> Malaxis latifolia </I> J. E. Sm.

Abstract

Root-tip chromosome investigations were carried out in Liparis siamensis Rolfe ex Downie and Malaxis latifolia J. E. Sm., aiming at finding suitable durations of root-tip sampling, pre-treatment and staining. It was found that suitable times of root-tip sampling were at 11.00 am in L. siamensis and 7.00 am in M. latifolia while that of pre-treatment was 1 hour and 1 hour 30 minutes in L. siamensis  and M. latifolia  respectively. As for staining, the duration was best at 30 minutes in both species. Chromosome counts revealed 2n = 42 in L. siamensis  and  2n = 40 in M. latifolia.