Chromosome Investigation of Amazon Lily (<I>Eucharis grandiflora </I> Planch. & Lind.)

Abstract

Root-tip preparation techniques for somatic chromosome investigation of Eucharis grandiflora Planch. & Lind.   were  studied.  The studies  comprised  of  1)  root-tip  sampling  duration at from 7.30 am to 4.30  pm  2)  pre-treatment  duration in  para-dichlorobenzene (PDB) of  6, 12,  24,  48  and  72 hour  and   3) staining duration in carbol fuchsin of  6, 12, 24, 48  and 72 hour.  The  results  showed  suitable technique  of  root-tip  preparation  for  chromosome investigation  being  sample  taking  at  9.30 am  followed  by  fixation  with  PDB  treatment  for  48  hours  and staining  with carbol  fuchsin for 12  hours. Chromosome  counts  revealed  2n=68.