Effects of Sucrose and Coconut Water on Growth and Development of <I>Brachycorythis</I> sp.
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Abstract
Experiments to find a culture medium supplemented with suitable sucrose and coconut water concentrations for growth of plant and tuber by culturing Brachycorythis sp. seedlings, 2 – 3 mm in size, in modified Vacin and Went (1949) (CMU1) medium with various sucrose concentrations, i.e. 0, 2 and 4% (w/v) and coconut water at 0, 7.5, 15.0, 22.5 and 30.0% (v/v) showed that the seedlings could grow and form tuber in the media with sucrose and/or coconut water at all levels. There was interaction between both factors. Coconut water promoted growth, whereas sucrose was essential for root formation. After culturing for 16 weeks, the seedlings cultured in the medium with 2% sucrose and 15.0% coconut water gave overall best growth and development.
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ธีรพล พรสวัสดิ์ชัย. 2535. ปัจจัยที่มีอิทธิพลต่อการงอกและการพัฒนาของโปรโตคอร์มของรองเท้านารีเหลืองปราจีน. วิทยานิพนธ์วิทยาศาสตร มหาบัณฑิต. มหาวิทยาลัยเชียงใหม่, เชียงใหม่. 160 หน้า.
Arditti, J. and R. Ernst. 1992. Micropropagation of Orchids. John Wiley & Sons Inc., New York. 682 pp.
Arditti, J., M.A. Clement, G. Fast, G. Hadley and R. Ernst. 1982. Orchid seed germination and seedling culture: A manual. pp. 243-370. In J. Arditti, (ed.). Orchid Biology Reviews and Perspective, II. Cornell University Press. New York.
Huang, L.C., C.J. Lin, C.I. Kuo, B.L. Huang and T. Murashige. 2001. Paphiopedilum cloning in vitro. Scientia Horticulturae 91(1-2): 111-121.
Ichihashi, S. and H. Hiraiwa. 1996. Effect of solidifiers, coconut water, and carbohydrate source on growth of embryogenic callus in Phalaenopsis and allied genera. J. Orchid Soc. India 10(1-2): 81-88.
Mathews, V.H. and P.S. Rao. 1980. In vitro multiplication of Vanda hybrids through tissue culture technique. Plant Science Lettera 17: 383-389.
Pan, R.C., Q.S. Ye and C.S. Hew. 1997. Physiology of Cymbidium sinense: A review. Scientia Horticulturae 70(2-3): 123-129.
Seidenfaden, G. 1977. Orchid Genera in Thailand V: Orchidoideae. Dansk Botanisk Arkiv 31(3): 1-149.
Sharma, S.K. and P. Tandon. 1990. Asymbiotic germination and seedling growth of Cymbidium elegans Lindl. and Coelogyne punctulata Lindl. as influenced by different carbon sources. J. Orchid Soc. India 4: 149-159.
Vacin, E. and F. Went. 1949. Some pH changes in nutrients sulutions. Bot. Gaz. 110: 605-613.