Genetic Relationships in Longan var. Daw as Revealed by Isozyme Analysis
Article Sidebar
Main Article Content
บทคัดย่อ
Twenty clones of longan var. Daw were fingerprinted, classified and evaluated for phylogenetic relationships using isozyme pattern analysis. Ten enzyme systems were screened. It is found that two enzyme systems failed to be stained in polyacrylamide gel including alcohol dehydrogenase (ADH) and isocitrate dehydrogenase (IDH). The eight enzyme systems that produced clear and reproducible isozyme patterns were selected, including acid phosphatase (ACP), aspartate amino transferase (AAT), esterase (EST), glucose-6-phosphate dehydrogenase (G6PD), glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase (G3PD), malate dehydrogenase (MDH) and peroxidase (POX). The number of schematic zymograms were 15, 13, 11, 19, 13, 11, 14 and 9, respectively with the total of 105. At 95% similarity, cluster analysis based on molecular data of band distribution in all samples showed 19 clusters and 1 individual not belonging to any of the clusters. Daw Bai Hod (DBH) and Daw Kan On (DKO) could not be separated with 95% similarity while the rest of the clones could be separated with similarity less than 95%. Daw Kan Kaeng (DKK) and Daw Nam Pueng (DNP) as well as Daw Luang (DLG) and Daw Sukhum (DSK) had 80% similarity. Daw Lum Nam Ping (DLP) and Daw Soy (DSY) had 68% similarity while Daw Nan Sao (DNS) and Daw Ta Noi (DTN) had 64% similarity. Daw Yod Kao (DYK) was the only individual that had 0% similarity and was completely different from the others.
Article Details
References
Bhat, K.V., S. R. Bhat and K. P. S. Chandel. 1992 a. Survey of isozymepolymorphism for clonal identification in Musa. I. Esterase, acid phosphatase and catalase. J. Hort. Sci. 67(6) 501-507.
Bhat, K. V., S. R. Bhat and K. P. S. Chandel. 1992 b. Survey of isozymepolymorphism for clonal identification in Musa. II. Peroxidase, superoxide dismutase, shikimate dehydrogenase and malate dehydrogenase. J. Hort. Sci. 67(6): 737-743.
DeWald, M. G., G. A. Moore and W. B. Sherman. 1988. Identification of pineapple cultivars by isozyme genotypes. J. Amer. Soc. Hort. Sci. 113 (6): 935-938.
Hiratsuka, S., K. Ichimura, E. Takahashi and N. Hirata. 1986. Analysis of protein in developing style and ovary with reference to self-incompatibility of Japanese pear. J. Japan. Soc. Hort. Sci. 55(2): 145-152.
Huang, H., F. Dane and J. D. Norton. 1994. Genetic analysis of 11 polymorphic isozyme loci in chestnut species and characterization of chestnut cultivars by multi-locus allozyme genotypes. J. Amer. Soc. Hort. Sci. 119 : 840 849.
Juengyusuk, P. 1998. Longan. p. 1-5. In Thai Longan Future in 2000.Office of Agriculture, Muang District, Lamphun. (in Thai).
Kanjana, P. 1998. Identification og Longan Varieties by Electrophoretic and Cytogenetic Methods. M.S. Thesis in Horticulture, Chiang Mai University, Chiang Mai. 122 p. (in Thai).
Kumar, S., V. P. Sharma and H. C. Kapoor. 1995. Isozymic identification of cultivars of ber (Zizyphus mauritiana Lamk.). J. Hort. Sci. 70(2): 303-306.
Kumnuan, P. 2001. Hybridization and Identification of Strawberry Hybrids by Morphological, Electrophoretic and Cytogenetic Methods. M.S. Thesis in Horticulture, Chiang Mai University Chiang Mai. 238 p. (in Thai).
Kuntapanom, P. and P. Sirikam. 2001. Genetic Diversity of Silverberry using Isozyme Patterns. Final Report submitted to Chiang Mai University, Chiang Mai. (in Thai).
Obara-Okeyo, P., K. Fujii and S. Kako. 1998. Isozyme variation in Cymbidium species (Orchidaceae). HortScience 33 (1); 133-135.
O'Brin, S. J. 1990. Genetic Maps. Cold Spring Harbor Press, Cold Spring Harbor, New York. Ramingwong, K. 2003. Systematic Pomology. Department of Horticulture, Faculty of Agriculture, Chiang Mai University, Chiang Mai. (in Thai).
Ramingwong, K. and K. Chiewsilp. 1994. Genetic Resources of Longan in Northern Thailand. Final Report submitted to Chiang Mai University, Chiang Mai. 76p.
Ramingwong, K., P. Sirikam and P. Kanjana. 1998. Identification of Longan Varieties by Morphological, Biochemical and Cytogenetic Methods. Final Report submitted to Chiang Mai University, Chiang Mai. 50 p.
Schmid, A.1980. Electrophoresis Techniques. Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok. 106. (in Thai).
Sokal, R.R. and P.H.A. Sneath. 1973. Principles of Numerical Taxonomy. Freeman, San Francisco. 573 p.
Subhadrabandhu, S. and S. Suriyapananont. 1998. Peroxidase isozyme patterns of some sweet Tamarind (Tamarindus indica L.) varieties in Thailand. J. ISSAAS 4 : 43-56.
Thongthieng, T. 2002. Strawberry Improvement and Their Identification using Molecular Markers. Ph.D. Thesis in Biotechnology, Chiang Mai University, Chiang Mai. 121 p.
Torres, A.M., R.K. Soost and T. Mau-Lastovicka. 1982. Citrus isozymes.J. Hered. 73: 335-339. Yapwattanaphun, C. 1998. Identification of Lychee Varieties by Morphological, Electrophoreticand Cytogenetic Methods. M.S. Thesis in Horticulture, Chiang Mai University, Chiang Mai. 109 p. (in Thai).