Detection of Viruses Caused Leaf Spot and Crinkle Leaf in Strawberry cv. Praratchatan 80 Growing in Fang District, Chiang Mai Province
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Abstract
Strawberry cultivation using stolon from repeatedly long-term mother plant is possibly accumulated with various viruses and affected to plant growth, flowering, fresh fruit yield, and qualities. The purpose of this research was to explore the infection of virus in strawberry cv. Praratchatan 80 in Ban Nong Tao and Ban Khop Dong (Mon Pin sub-district) and Ban Mae Ngon Khi Lek (Mae Ngon sub-district), Fang district, Chiang Mai province. A total of 175 strawberry leaf samples were collected from 43 symptomatic samples and 132 non-symptomatic samples. Virus detection was conducted by using a reverse transcription-polymerase chain reaction (RT-PCR) technique. The result showed that there were 134 samples (76.57 %) of virus-infected strawberry. Of the detection number, 92 samples (52.57 %) were identified as a single infection, including 80 samples (45.71 %) of strawberry latent ringspot virus (SLRSV) and 12 samples (6.86 %) of strawberry crinkle virus (SCV). Mixed infection of SLRSV and SCV was also detected at 42 samples (24.0%). Detected samples in each area were found 58 samples (77.33 %) in Ban Nong Tao, 59 samples (78.67 %) in Ban Khop Dong and 17 samples (68 %) in Ban Mae Ngon Khi Lek. The nucleotide sequences of DNA synthesis for the CP gene – RNA 2 (SLRSV) and L protein gene (SCV) regions were obtained from PCR products with sizes of 497 bp and 345 bp, respectively. They were then analyzed and compared to the GenBank database. Results showed that the nucleotide sequence of 497 bp was similar to 5 isolates of SLRSV (Mint 454, NCGR MEN, Eg, Mint-CP2 and W16) at 89.21 - 99.8% identity and the sequence of 345 bp was similar to 4 isolates of SCV (1554, KG, H2399 and H2395) at 94.4 - 100% identity. To the best of our knowledge, this is the first report of SLRSV and SCV virus infecting strawberry in Thailand.
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