The Development of Moisturizing Product from Okra (<I>Abelmoschus esculentus</I> (L.) Moench) Extract

Main Article Content

Phatchareewan Bhaokam
Trinlada Saengtong
Nitchanan Suchonwattanakul
Mathukorn Sainakham

Abstract

The aim of this study was to extract and examine biological activity of okra (Abelmoschus esculentus L.) pod for the development of moisturizing product. In this study, the okra pod was extracted by water and ethanol at various concentrations with maceration and reflux. After that, crude extract was determined polysaccharide and total phenolic content, free radical scavenging and lipid peroxidation activities, formulated the emulsion-gel containing okra pod extract formulated for stability evaluation, determined skin irritation by patch-tested and moisturizing effect in women volunteers skin. The results showed that dry okra extracted using 50% ethanol by maceration process (MTD) gave % yield at 27.50 %, total polysaccharides at 5.58 ± 0.03 mg glucose/g, total phenolic compounds at 1.24 ± 0.01 mg GAE/g, SC50 value of 0.59 ± 0.07 mg/ml and IPC50 value of 0.02 ± 0.02 mg/ml. MTD extract was selected to formulate emulsion-gel products which showed high stability, no irritation and increasing the hydration of women volunteer skin for 90 minutes. Therefore, cosmetic products containing okra pod extract have the potential for development of skin moisturizer.

Article Details

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Research Articles

References

Chansukh, K. 2019. Isolation, separation and identification of active principles from medicinal plants. (Online). Available: elfit.ssru.ac.th/kittsak_ja/pluginfile.php/98/block_html/content/4-Isolation Separation and identification of active principles from medicinal plants-kitthisak.pdf (October 21, 2019). (in Thai)

Chen, Y., M.F. Wang, R.T. Rosen and C.T. Ho. 1999. 2,2-diphenyl-1-picrylhydrazyl radical scavenging active components from Polygonum multiforum Thumb. Journal of Agricultural and Food Chemistry 47(6): 2226-2228.

di Nardo, A., K. Sugino, P. Wertz, J. Ademola and H.I. Maibach. 1996. Sodium lauryl sulfate (SLS) induced irritant contact dermatitis: a correlation study between ceramides and in vivo parameters of irritation. Contact Dermatitis 35(2): 86-91.

Fan, S., Y. Zhang, Q. Sun, L. Yu, M. Li, B. Zheng, X. Wu, B. Yang, Y. Li and C. Huang. 2014. Extract of okra lowers blood glucose and serum lipids in high-fat diet-induced obese C57BL/6 mice. Journal of Nutritional Biochemistry 25(7): 702-709.

Gülçin, I. 2006. Antioxidant activity of caffeic acid (3, 4-dihydroxycinnamic acid). Toxicology 217(2-3): 213-220.

Huang, C.K. and T.A. Miller. 2007. The truth about over-the-counter topical anti-aging products: A comprehensive review. Aesthetic Surgery Journal 27(4): 402-412.

Huda-Faujan, N., Z.A. Rahim, M.M. Rehan and F.B.H. Ahmad. 2015. Comparative analysis of phenolic content and antioxidative activities of eight Malaysian traditional vegetables. Malaysian Journal of Analytical Sciences 19(3): 611-624.

Jaipakdee, N., K. Trakanchaiwong and E. Limpongsa. 2015. Evaluation of physical stability, efficacy and preference of anticellulite cream containing natural compounds. Isan Journal of Pharmaceutical Sciences 11(2): 55-70. (in Thai)

Jia, G.T. and H.Q. Zhao. 2015. Extraction and Antioxidant Activity of Polysaccharide from Salvia miltiorrhiza. pp. 106-109. In: Proceedings of International Conference on Chemical, Material and Food Engineering. Atlantis Press, Paris.

Jia, L., H.F. Li and L.L. Jing 2011. Chemical constituents in n-butanol extract of Abelmoschus esculentus L. Chinese Traditional and Herbal Drugs 41(11): 1771-1773.

Knight, J.A. and R.P. Voorhees. 1990. Peroxidation of linolenic acid—catalysis by transition metal ions. Annals of Clinical and Laboratory Science 20(5): 347-352.

Kanlayavattanakul, M., C. Rodchuea and N. Lourith. 2012. Moisturizing effect of alcohol-based hand rub containing okra polysaccharide. International Journal of Cosmetic Science 34(3): 280-283.

Limpongsa, E., N. Jaipakdee and S. Deeseenthum. 2014. Formulation and evaluation of facial masks prepared from Thai jasmine rice. KKU Research Journal 19(6): 905- 915. (in Thai)

Manosroi, A., P. Jantrawat, M. Sainakham, W. Manosroi and J. Manosroi. 2012. Anticancer activities of the extract from Longkong (Lansium domesticum) young fruits. Pharmaceutical Biology 50(11): 1397-1407.

Manosroi, A., M. Sainakham, C. Chankhampan, W. Manosroi and J. Manosroi. 2016. In vitro anti-cancer of Job’s tears (Coix lachryma-jobi Linn.) extracts on human colon adenocarcinoma. Saudi Journal of Biological Sciences 23(2): 248-256.

Roy, A., S.L. Shrivastava and S.M. Mandal. 2014. Functional properties of okra Abelmoschus esculentus L. (Moench): traditional claims and scientific evidences. Plant Science Today 1(3): 121-130.

Visioli, F. and C. Galli. 1998. The effect of minor constituent of olive oil on cardiovascular disease: new findings. Nutrition Reviews. 56(5): 142-147.

Yuan, Q., S. Lin, Y. Fu, X.R. Nie, W. Liu, Y. Su, Q.H. Han, L. Zhao, Q. Zhang, D.R. Lin, W. Qin and D.T. Wu. 2019. Effects of extraction methods on the physicochemical characteristics and biological activities of polysaccharides from okra (Abelmoschus esculentus). International Journal of Biological Macromolecules 127: 178-186.

Zhang, Q., J. Zhang, J. Shen, A. Silva, D.A. Dennis and C.J. Barrow. 2006. A simple 96-well microplate method for estimation of total polyphenol content in seaweeds. Journal of Applied Phycology 18(3-5): 445-450.

Zou, P., X. Yang, W.W. Huang, H.T. Zhao, J. Wang, R.B. Xu, X.L. Hu, S.Y. Shen and D. Qin. 2013. Characterization and bioactivity of polysaccharides obtained from pine cones of Pinus koraiensis by graded ethanol precipitation. Molecules 18(8): 9933-9948.