Formulation Development of <I> Metarhizium anisopliae</I> (NU-048) Concentrated Bioproduct to Control Brown Planthopper
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Abstract
The objective of this work was to develop the effective formulations of Metarhizium anisopliae (NU-048) concentrated bioproduct preserved in accelerated condition at high temperature (35°C) to control brown planthopper. The bioproduct were prepared using the mixture of wheat flour, sand and maltose as the base and added with 10 different nitrogen sources: monosodium glutamate, L-glutamine, L-asparagine monohydrate, L-alanine, L-cysteine, L-leucine, L-arginine, casein, potassium nitrate and urea in each formulation. All products were kept at 35°C for 6 weeks, the germination ability of fungal conidia was determined every week and their growth abilities in growth medium and efficacy to control brown planthopper were evaluated at 6 weeks. The results found that the germination ability of M. anisopliae conidia in all formulas was varied on nitrogen sources and shelf life. The highest germination rate and growth on growth medium was found from the formula that added L-arginine at 33% with the highest amount of conidia formation on growth medium at the concentration of 2.1 x 1010 conidia/g after 14 days of incubation. The highest mortality rate of brown plant hopper at 93-100 % was found on the formulas that added L-arginine and monosodium glutamate at 4 days after application with LT50 at 2.45 and 2.40 days, respectively.
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References
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