Chromosome Doubling of <I>Habenaria erichmichaelii Christenson</I> Using Colchicine <I>in Vitro</I>
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Abstract
Terrestrial orchids, Habenaria erichmichelii Christenson is one of the most beautiful terrestrial orchids. Plant is compact with beautiful flowers and has great potential for commercial use as potted plants. Thus, effects of using colchicine in vitro was carried out to double chromosome number of Habenaria erichmichelii Christenson by soaking 1 - month old germinated protocorms in colchicine at different concentrations, 0.0, 0.01, 0.025, 0.05 and 0.10 % (w / v) for 5 and 10 days. The protocorms were cultured on PGR - free MS medium for 4 months. The results showed that the chromosome number from root - tips of the control group were diploid with 2n = 2x = 42, the chromosome number of those treated with 0.05 % and 0.10 % (w / v) colchicine for 10 days were mixoploid with 2n = 2x = 42 and 2n = 4x = 84. Moreover, soaking in 0.01 % colchicine for 10 days gave better survival rate compared with colchicine at concentration of 0.05 % for 10 days, whereas plant height tended to decrease, stomata density was decreased, and stomata size was increased.
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Atichart, P. 2014. Effect of concentration and duration of colchicine on survival rate and cell characteristic of Dendrobium chrysotoxum Lindl. protocorm. KKU Science Journal 42(2): 372-379. (in Thai)
Caperta, A.D., M. Delgado, F. Ressurreicao, A. Meister, R.N. Jones, W. Viegas and A. Houben. 2006. Colchicine-induced polyploidization depends on tubulin polymerization in C-metaphase cells. Protoplasma 227(2-4): 147-153.
Chinachit, W. and S. Sreemaung. 2008. Colchicine affecting the alteration of ploidy level in plantlets of Eulophia andamanensis Reichb. f. Agricultural Science Journal 39(3) (Suppl.): 275-277. (in Thai)
Fehr, W.R. 1987. Principles of Cultivar Development Vol.1. New York: Macmillan.
Hawkes, A.D. 1965. Encyclopedia of Cultivated Orchids: An Illustrates, Descriptive Manual of the Members of the Orchidaceae Currently in Cultivation. Faber and Faber, London. 602 p.
Kawchadee, C., W. Bundithya, C. Sotthikul and N. Potapohn. 2012. Interspecific and intergeneric crossability of some Habenaria and Pecteilis, terrestrial orchids. Journal of Agriculture 28(3): 263-272. (in Thai)
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.
Petbanna, U. 2011. Chromosome doubling of Spathoglottis plicata Blume var. alba using colchicine and oryzalin in vitro. M. Sc. Thesis. Walailak University, Nakhon Si Thammarat. 105 p. (in Thai)
Petchang, R. 2010. The effects of colchicine concentration and treatment duration on growth and chromosome number in Dendrobium draconis Rchb. f. Journal of Science and Technology Mahasarakham University 29(4): 413-419. (in Thai)
Potapohn, N. 2005. Orchidology 1. Handout. Department of Horticulture, Faculty of Agriculture, Chiang Mai University, Chiang Mai. 215 p. (in Thai)
Saikum, C., K. Kunasakdakul and N. Potapohn. 2020. Crossability and in vitro seed germination of some terrestrial orchid species of genus Habenaria and Pecteilis. Journal of Agriculture 36(1): 47-58. (in Thai)
Sarathum, S., M. Hegele, S. Tantiviwat and M. Nanakorn. 2010. Effect of concentration and duration of colchicine treatment on polyploidy induction in Dendrobium scabrilingue L. European Journal of Horticultural Science 75(3): 123-127.
Silva, P.A.K.X., S.C. Jacques and M.H.B. Zanettini. 2000. Induction and identrification of polyploids in Cattleya intermedia Lindl. (Orchidacea) by in vitro techniques. Ciencia Rural, Santa Maria 30(1): 105-111.
Sitthisatchatham, S. 2007. Wild Orchid of Thailand. Home and Garden, Bangkok. 495 p. (in Thai)
Teo, C.K.H. 1985, Native Orchids of Peninsula Malaysia. Kyodo-Shing Loong Printing Indrustries (Pte) Ltd., Kuala Lumpur. 119 p.
Thaithong, O. 2005. Orchids of Thailand. Home and Garden, Bangkok. 461 p. (in Thai)
Tilarux, P. and S. Prasertsiriwat. 2014. Effects of colchicine in the induction of polyploidy of Dendrobium friedericksianum Rchb.f. (var. oculatum). Journal of Science and Technology, Ubon Ratchathani University 16(1): 61-68. (in Thai)
Tipvaree, V. 2009. Effects of colchicine on growth and polyploid induction of Spathoglottis plicata. M. S. Thesis. Chiang Mai University, Chiang Mai. 82 p. (in Thai)
