Study on Genetic Relationship of <I>Dimocarpus longan</I> Using ID-RAPD Technique
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Abstract
Longan (Dimocarpus longan Lour.) is an economically important crop. There are numerous longan varieties grown in the northern part of Thailand. Currently, molecular techniques are used to study the genetic relationship of longan. In this study, Intron Derived Random Amplified Polymorphic DNA (ID- RAPD) was used to investigate the genetic relationship of 25 varieties of longan which were collected at Maejo University. Fifty amplified DNA fragments were obtained from ten ID-RAPD primers. Forty-five of 50 amplified bands were polymorphism (90%). Genetic similarity and cluster analysis was performed using NTSYSpc program. The genetic similarity index ranged from 0.46-0.98. The dendrogram showed that 25 varieties of longan were classified into 3 groups with a 0.74 similarity index. Moreover, Daw varieties were clearly separated from other varieties.
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