Effects of Sucrose, Activated Carbon, and Light Duration on <I>In Vitro</I> Microrhizome Formation of Ginger
Article Sidebar
Published:
Jul 24, 2020
Keywords:
Sucrose activated carbon light duration microrhizome formation In Vitro Zingiber officinale Roscoe
Main Article Content
Abstract
In vitro microrhizome formation of ginger was induced by culturing pseudostem explants onto modified MS agar with 0.5 mgL-1 BA. After 3 months, it was found that sucrose concentration and light duration affected microrhizome formation and plant growth. Ten percent sucrose caused a lower height and number of leaves compared to 3-8% sucrose. Light duration of 16 and 24 hrs promoted a higher number of leaves compared to 0 hr of light duration. Application of 6-10% sucrose under 16 and 24 hrs of light duration improved microrhizome formation. In addition, 8% sucrose application under 16 hrs of light duration was the most suitable for microrhizome formation, giving microrhizoming ratio (the ratio of the greatest diameter near the base to the diameter of the pseudostem) of 3.0 and microrhizome formation of 100%. Application of activated carbon did not affect microrhizome formation, and decreased number of shoots and leaves.
Article Details
Section
Research Articles
References
ธีรพล พรสวัสดิ์ชัย และ พิมพ์ใจ อาภาวัชรุตม์. 2550. ผลของน้ำตาลซูโครสต่อการเจริญเติบโตและการสร้างหัวในสภาพหลอดแก้วของกล้วยไม้ดินนางอั้วสาคริก. วารสารเกษตร 23(3): 197-205.
อบเชย อิ่มสบาย มณทิพย์ สิทธิพิพัฒน์ นฤวรรณ รักสกุล และ จันทร์ทิพย์ แซ่เอี้ยว. 2540. ขิง-ข่า ตำรับอาหารเพื่อสุขภาพ. สำนักพิมพ์แสงแดด จำกัด, กรุงเทพฯ. 111 หน้า.
Archana, C. P., S. P. Geetha and I. Balachandran. 2013. Microrhizome and minirhizome production in three high yielding cultivars of ginger (Zingiber officinale Rosc.). International Journal of Current Microbio- logy and Applied Sciences 2(10): 477-484.
Babu, K. N., K. Samsudeen and M. J. Ratnambal. 1992. In vitro plant regeneration from leaf-derived callus in ginger (Zingiber officinale Rosc.). Plant Cell Tissue and Organ Culture 29(2): 71-74.
Boggetti, B., J. Jasik and S. H. Mantell. 1999. In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse-raised plants. Plant Cell Reports 51: 456-461.
Garner, N. and J. Blake. 1989. The Induction and development of potato microtubers in vitro on media free of growth regulating substances. Annals of Botany 63(6): 663-674.
Han, B. H., B. W. Yae, H. J. Yu and K. Y. Peak. 2005. Improvement of in vitro micropropagation of Lilium oriental hybrid ‘Casablanca’ by the formation of shoots with abnormally swollen basal plates. Scientia Horticulturae 103: 351-359.
Haq, F., Faruque, S. M., Islam, S., Ali, E., 1986. Studies on Zingiber officinale Roscoe part 1: Chemical investigation of the rhizome. Bangladesh Journal of Scientific and Industrial Research 21(1-4): 61-69.
Islam, M. A., K. Kloppstech and H. J. Jacobsen. 2004. Efficient procedure for in vitro microrhizome in Curcuma longa L. (Zingiberacea) - A medicinal plant of tropical asia. Plant Tissue Culture 14(2): 123-134.
Kackar, A., S. R. Bhat, K. P. S. Chandel and S. K. Malik.1993. Plant regeneration via somatic embryogenesis in ginger. Plant Cell Tissue and Organ Culture 32(3): 289-292.
Kavyashree, R. 2009. Efficient in vitro protocol for clonal multiplication of ginger- var. Varada. Indian Journal of Biotechnology 8: 328-331.
Lincy, A. and B. Sasikumar. 2010. Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies. Turkish Journal of Botany 34: 21-39.
Mulin, M. and A. Bellio-Spataru. 2000. Organogenesis from hypocotyl thin cell layers of Lupinus mutabilis and Lupinus albus. Plant Growth Regulation 30: 177-183.
Nayak, S. 2000. In vitro multiplication and microrhizome induction in curcuma aromatic Salisb. Plant Growth Regulation 32: 41-47.
Nayak, S. and P. K. Naik. 2006. Factors effecting in vitro micropropagation formation and growth in Curcuma longa L. and improved field performance of micropropagated plants. Science Asia. 32: 31-37.
Nissen, S. J. and E. G. Suttur. 1990. Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience 25: 800-802.
Pan, M. J. and J. Staden. 1998. The use of charcoal in in vitro culture - A review. Plant Growth Regulation 26: 155-163.
Pandey, Y. R., C. Sagwansupyakorn, O. Sahavacharin and N. Thaveechai. 1997. In vitro propagaton of ginger (Zingiber officinale Roscoe). Kasetsart Journal (Natural Science) 31: 81-86.
Peak, N. Y. and E. C. Yeung. 1991. The effects of 1-napthalene acetic acid and N6-benzyladenine on the growth of Cymbidium forrestii rhizomes in vitro. Plant Cell Tissue and Organ Culture 24: 65-71.
Ravindran, P. K. and N. K. Babu. 2005. Introduction. pp. 1-14. In: Ravindran, P. N. and N. K. Babu (EDs). Ginger: The Genus Zingiber. CRC Press, Florida.
Rout, G. R., S. K. Palai, S. Samamtaray and P. Das. 2001. Efficient of growth regulator and culture conditions on shoot multiplication and rhizome formation in ginger (Zingiber officinale Rosc.) in vitro. In Vitro Cellular & Developmental Biology-Plant. 37: 814-819.
Saingproa, B. and K. Kanchanapoom. 1997. Clonal propagation through multiple shoot formation from ginger (Zingiber officinale Roscoe) callus and buds. Suranaree Journal of Science and Technology 4: 1-5.
Saos, F. Le Guen-le, A. Hourmant, F. Esnault and J. E. Chauvin. 2001. In vitro bulb development in Shallot (Allium cepa L. Aggregatum Group): Effect of anti-gibberrellins, sucrose and light. Annals of Botany 89: 419-425.
Sharma, T. R. and B. M. Singh. 1995. In vitro microrhizome production in Zingiber officinale Rosc. Plant Cell Reports 15(3-4): 274-277.
Sharma, T. R. and B. M. Singh. 1997. High-frequency in vitro multiplication of disease-free Zingiber officinale Rosc. Plant Cell Reports 17(1): 68-72.
Sheelavantmath, S. S., H. N. Murthy, A. N. Pyati, H. G. Ashok Kumar and B. V. Ravishankar. 2000. In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell Tissue and Organ Culture 60: 151-154.
Sultana, A., L. Hassan, S. D. Ahmad, A. H. Shah, F. Batool, M. A. Islam, R. Rahman and s. Moonmoon. 2009. In vitro regeneration of ginger using leaf, shoot tip and root explant. Pakistan Journal of Botany 41(4): 1667-1676.
Thomas, T. D. 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advances 26: 618-631.
Tyagi, R. K., A. Agrawal and A. Yusuf. 2006. Conservation of Zingiber germplasm through in vitro rhizome formation. Scientia Horticulturae 108: 210-219.
อบเชย อิ่มสบาย มณทิพย์ สิทธิพิพัฒน์ นฤวรรณ รักสกุล และ จันทร์ทิพย์ แซ่เอี้ยว. 2540. ขิง-ข่า ตำรับอาหารเพื่อสุขภาพ. สำนักพิมพ์แสงแดด จำกัด, กรุงเทพฯ. 111 หน้า.
Archana, C. P., S. P. Geetha and I. Balachandran. 2013. Microrhizome and minirhizome production in three high yielding cultivars of ginger (Zingiber officinale Rosc.). International Journal of Current Microbio- logy and Applied Sciences 2(10): 477-484.
Babu, K. N., K. Samsudeen and M. J. Ratnambal. 1992. In vitro plant regeneration from leaf-derived callus in ginger (Zingiber officinale Rosc.). Plant Cell Tissue and Organ Culture 29(2): 71-74.
Boggetti, B., J. Jasik and S. H. Mantell. 1999. In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse-raised plants. Plant Cell Reports 51: 456-461.
Garner, N. and J. Blake. 1989. The Induction and development of potato microtubers in vitro on media free of growth regulating substances. Annals of Botany 63(6): 663-674.
Han, B. H., B. W. Yae, H. J. Yu and K. Y. Peak. 2005. Improvement of in vitro micropropagation of Lilium oriental hybrid ‘Casablanca’ by the formation of shoots with abnormally swollen basal plates. Scientia Horticulturae 103: 351-359.
Haq, F., Faruque, S. M., Islam, S., Ali, E., 1986. Studies on Zingiber officinale Roscoe part 1: Chemical investigation of the rhizome. Bangladesh Journal of Scientific and Industrial Research 21(1-4): 61-69.
Islam, M. A., K. Kloppstech and H. J. Jacobsen. 2004. Efficient procedure for in vitro microrhizome in Curcuma longa L. (Zingiberacea) - A medicinal plant of tropical asia. Plant Tissue Culture 14(2): 123-134.
Kackar, A., S. R. Bhat, K. P. S. Chandel and S. K. Malik.1993. Plant regeneration via somatic embryogenesis in ginger. Plant Cell Tissue and Organ Culture 32(3): 289-292.
Kavyashree, R. 2009. Efficient in vitro protocol for clonal multiplication of ginger- var. Varada. Indian Journal of Biotechnology 8: 328-331.
Lincy, A. and B. Sasikumar. 2010. Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies. Turkish Journal of Botany 34: 21-39.
Mulin, M. and A. Bellio-Spataru. 2000. Organogenesis from hypocotyl thin cell layers of Lupinus mutabilis and Lupinus albus. Plant Growth Regulation 30: 177-183.
Nayak, S. 2000. In vitro multiplication and microrhizome induction in curcuma aromatic Salisb. Plant Growth Regulation 32: 41-47.
Nayak, S. and P. K. Naik. 2006. Factors effecting in vitro micropropagation formation and growth in Curcuma longa L. and improved field performance of micropropagated plants. Science Asia. 32: 31-37.
Nissen, S. J. and E. G. Suttur. 1990. Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience 25: 800-802.
Pan, M. J. and J. Staden. 1998. The use of charcoal in in vitro culture - A review. Plant Growth Regulation 26: 155-163.
Pandey, Y. R., C. Sagwansupyakorn, O. Sahavacharin and N. Thaveechai. 1997. In vitro propagaton of ginger (Zingiber officinale Roscoe). Kasetsart Journal (Natural Science) 31: 81-86.
Peak, N. Y. and E. C. Yeung. 1991. The effects of 1-napthalene acetic acid and N6-benzyladenine on the growth of Cymbidium forrestii rhizomes in vitro. Plant Cell Tissue and Organ Culture 24: 65-71.
Ravindran, P. K. and N. K. Babu. 2005. Introduction. pp. 1-14. In: Ravindran, P. N. and N. K. Babu (EDs). Ginger: The Genus Zingiber. CRC Press, Florida.
Rout, G. R., S. K. Palai, S. Samamtaray and P. Das. 2001. Efficient of growth regulator and culture conditions on shoot multiplication and rhizome formation in ginger (Zingiber officinale Rosc.) in vitro. In Vitro Cellular & Developmental Biology-Plant. 37: 814-819.
Saingproa, B. and K. Kanchanapoom. 1997. Clonal propagation through multiple shoot formation from ginger (Zingiber officinale Roscoe) callus and buds. Suranaree Journal of Science and Technology 4: 1-5.
Saos, F. Le Guen-le, A. Hourmant, F. Esnault and J. E. Chauvin. 2001. In vitro bulb development in Shallot (Allium cepa L. Aggregatum Group): Effect of anti-gibberrellins, sucrose and light. Annals of Botany 89: 419-425.
Sharma, T. R. and B. M. Singh. 1995. In vitro microrhizome production in Zingiber officinale Rosc. Plant Cell Reports 15(3-4): 274-277.
Sharma, T. R. and B. M. Singh. 1997. High-frequency in vitro multiplication of disease-free Zingiber officinale Rosc. Plant Cell Reports 17(1): 68-72.
Sheelavantmath, S. S., H. N. Murthy, A. N. Pyati, H. G. Ashok Kumar and B. V. Ravishankar. 2000. In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell Tissue and Organ Culture 60: 151-154.
Sultana, A., L. Hassan, S. D. Ahmad, A. H. Shah, F. Batool, M. A. Islam, R. Rahman and s. Moonmoon. 2009. In vitro regeneration of ginger using leaf, shoot tip and root explant. Pakistan Journal of Botany 41(4): 1667-1676.
Thomas, T. D. 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advances 26: 618-631.
Tyagi, R. K., A. Agrawal and A. Yusuf. 2006. Conservation of Zingiber germplasm through in vitro rhizome formation. Scientia Horticulturae 108: 210-219.
