Isolation and Identification of Citrus Nematode in Mae Rim and San Sai Districts, Chiang Mai Province

Abstract

During September to October 2005, isolation and identification of plant parasitic nematodes were conducted highland (990 m from mean sea level) and lowland (340 m from mean sea level) citrus plantations at Pong Yaeng subdistrict, Mae Rim district and Mae Faek subdistrict, San Sai district, Chiang Mai province respectively. At each site, 25 soil samples collected at 3-8 inches deep were mixed to gain 2.0 kilograms soil. In laboratory, the samples were isolated by Cobb sieving and gravity method combined with Baerman funnel method. Three species of plant parasitic nematode, Helicotylenchus sp., Rotylenchulus sp. and Tylenchorhynchus sp. and 4 species of free living nematode, Mononchus sp., Rhabditis sp., Cephalobus sp. and Dorylaimus sp. were found in highland. While in lowland, 4 species of plant parasitic nematode, Helicotylenchus sp., Criconemella sp., Rotylenchulus sp. and Tylenchorhynchus sp. and 3 species of free living nematode, Mononchus sp., Rhabditis sp. and Dorylaimus sp. were found in soil.