Somatic Embryo Induction in Vetiver Grass (<I>Vetiveria zizanioides </I> Nash) var. Songkhla 3
Article Sidebar
Main Article Content
Abstract
Somatic embryo induction and maturation of vetiver grass var. Songkla 3 was carried out using calli established from young leaf. The calli were cultured on Murashige and Skoog (MS) medium supplemented with various kinds and concentrations of plant growth regulators. MS supplemented with 2, 4 dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA) at the same concentration (1 mg/l) gave the highest ratio (90.91%) and highest number of somatic embryo at globular stage (2.71 nodules/callus) and heart shape stage (2.56%). MS medium with reduced concentration (1/4MS) gave green spot on somatic embryo. Culturing embryogenic callus in solid medium for a week followed by MS liquid medium with 1 mg/l 2,4-D and 1,000 mg/l casein hydrolysate gave the highest somatic embryo from heart to torpedo shape at 26.79%
Article Details
References
กรมพัฒนาที่ดิน. 2541. ความรู้เรื่องหญ้าแฝก. กระทรวงเกษตรและสหกรณ์. 115 หน้า.
Ipekci, Z. and N. Gozukirmizi. 2003. Direct somatic embryogenesis and synthetic seed production from Paulownia elongata. Plant Cell Reports 22: 16-24.
Leupin, R.E., M. Leupin, C. Ehret, K.H. Erismann and B. Witholt. 2000. Compact callus induction and plant regeneration of a non-flowering vetiver from Java. Plant Cell, Tissue and Organ Culture 62: 115-123.
Mucciarelli, M., M. Gallino, S. Scannerini and M. Maffei. 1993. Callus induction and plant regeneration in Vetiveria zizanioides. Plant Cell, Tissue and Organ Culture 35: 267-271.
Nanakorn, M., S. Surawattananon, C. Wongwattana, K. Namwongprom and S. Suwannachitr. 1998. In vitro induction of salt tolerance in grass (Vetiveria zizanioides Nash). J. Weed Sci. Tech. 43: 134-137.
Pareek, A. and S.L. Kothari. 2003. Direct somatic embryogenesis and plant regeneration from leaf cultures of ornamental species of Dianthus. Scientia Horticulturae 98: 449-459.
Sahrawat, A. K. and S. Chand. 2004. High frequency plant regeneration from coleoptile tissue of barley (Hordeum vulgare L.). Plant Science 167: 27-34.
Suzuki, S., M. Oota, and M. Nakano. 2002. Embryogenic callus induction from leaf explants of the Liliaceous ornamental plant, Agapanthus praecox ssp. orientalis (Leighton) Leighton: Histological study and response to selective agents. Scientia Horticulturae 95: 123-132.
Te-chato, S., H. Aslan and Y. Ibrohem. 2003. Improve callus induction and embryogenic callus formation from culture young leaves of oil palm seedling. Thai J. Agric. Sci. 35: 407-143.
Vietmeyer, N. D. 1996. Organizing vetiver’s next steps to global acceptance. pp. 18-26. In: N. Chomchalow and H.V. Henle (eds.). Proceedings of the First International Conference on Vetiver a Miracle Grass. The Chaipattana Foundation, Bangkok.
Vietmeyer, N. D. and F. R. Ruskin. 1993. Vetiver Grass: A Thin Green Line Against Erosion. National Academy Press, Washington, D. C.
Wang, Z. Y., J. Bell and A. Hopkins. 2003. Establishment of a plant regeneration system for wheatgrasses (Thinopyrum, Agropyron and Pascopyrum). Plant Cell, Tissue and Organ Culture 73: 265-273.
Yantcheva, A. A., M. Vlahova and A. Antanassov. 1998. Direct somatic embryogenesis and plant regeneration of carnation (Dianthus caryophyllus L.). Plant Cell Reports 18: 148-153.
