Histological Study on <I>In Vitro </I>Tuberization of <I>Pecteilis sagarikii </I>Seidenf.

Abstract

Histological changes on in vitro tuberization of Pecteilis sagarikii Seidenf. seedlings were determined by using paraffin embedding technique. Various stages of seedlings were used. It was found that after leaf primordia occurred, the meristematic cells divided and grew into the opposite direction of its protocorm, then extended straight down until it reached an optimal depth. Subsequently, the meristematic cells produced new leaf primordia covering a meristem which will be a new bud for the next growing season. At the same time, the lower part of the meristematic cells divided and continued to grow straight down to increase the tuber length and tuber width until a complete tuber was obtained.