Suitable Extraction Buffer for Producing Isozyme Patterns of Some Plicated-leaf Terrestrial Orchids.

Abstract

A study was carried out to produce isozyme patterns from 10 plicated-leaf terrestrial orchid species in 6 genera by polyacrylamide gel electrophoresis (PAGE) using 0.5 g young leaf, 1.5 ml extraction buffer in 4 different extraction buffers developed by Gottlieb, Apavatjrut et al., Obera-Okeyo et al. and Sharma & Jones. When 10% separating gel was used and the four enzymes were tested, i.e. Esterase (EST), Glutamate oxaloacetate transaminase (GOT), Leucine amino peptidase (LAP) and Shikimate dehydrogenase (SKD), it showed that Apavatjrut et al. solution yielded the best isozyme bands.