Effects of T - 2 Toxin on Cytotoxicity and Apoptosis of Embryonic Fibroblast Cells Obtained from Native Chicken
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Abstract
The objective of this study was to determine the toxicity of T - 2 toxin in poultry. Chicken embryonic fibroblast (CEF) cells were derived from crossbred native chickens and used as a model for cytotoxicity evaluation of T - 2 toxins at various concentrations (0, 0.01, 0.1, 1, and 10 µg / ml) for 24 and 48 hours. Cytotoxicity of CEF was increased when exposed to increasing concentrations of T - 2 toxin. Cell viability of CEF was lower than 40 % and 20 % for all concentrations of T - 2 toxin at 24 and 48 hours, respectively. CEF cells were induced to death and mostly in the early apoptosis phase (62.27 %) by T - 2 toxin. The results indicated that crossbred native chickens were less resistant and more sensitive to T - 2 toxin. Therefore, the contamination caused by T - 2 toxin should be monitored in poultry feed as it may cause disease in chickens.
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