Effect of Gamma Oryzanol on Immune Response in Male Mice

Abstract

γ-oryzanol is  antioxidant with immune system improvement efficiency. The study aimed to prove this hypothesis and finding the effective level by investigate the effect of four levels (0, 280, 800 and 1340 mg per kg) of pure γ -oryzanol supplementation to mice basal diet on Bovine Serum Albumin (BSA) antibody titer. A complete randomized design was applied to the experiment. 32 Institute of Cancer Research (IRC) male mice, approximately 6 weeks old, were randomly grouped into four mice per pen. There were 2 pens per treatment. Feed and water were given ad libitum. Antibody titer was evaluated first and followed by immunizing with BSA for 42 trial days during the experiment. On the first day of trial all mice were given BSA immunization and boosted two times at day 14, 28 respectively. Mice serum were collected before immunization on day 1, 10, 14, 21, 28, 42 and stored at -20°C. Evaluated IgG titer by Enzyme-Linked Immunosorbent Assay (ELISA). The results showed that no significant differences among treatments on day 0 (p>0.05). The mean log₂ IgG titer of mice on the γ-oryzanol supplemented groups developed significantly higher than control group (p<0.05). Dietary γ-oryzanol level ranging from 800-1340 mg per kg significantly increased antibody response to BSA (p<0.05).