Variation in Isozyme Characteristics of Selected Clones of Mango cv. Kaew

Abstract

52 selected Kaew mango clones from eight provinces of the Upper North were studied in order to compare isozyme characteristics for the identification purpose. Isozyme characteristics were studied by polyacrylamide gel electrophoresis technique. Enzyme from seven month-old leaves was extracted by Tris-buffer 0.1 M, pH 8.2. Twenty-two percent of gel concentration was suitable for acid phosphatase and esterase, while 7.5 percent concentration was suitable for peroxidase. It was found that the three isozyme systems: acid phosphatase, esterase and peroxidase separately could identify 52 clones into 10, 4 and 15 groups respectively. Using the combination of three isozyme systems, the 52 clones could be grouped into 20 clones and other 9 groups.