Interspecific Hybridization of <I>Pleurotus sajor – caju</I> (Fr.) Singer and <I>Pleurotus pulmonarius</I>

Abstract

Interspecific breeding between Pleurotus sajor-caju and P. pulmonarius by mon-mon crossing was studied, ahybrid that gave the highest yield with brittle fruit body was HM9. Yield harvested for 35 days. Di-mon crossing between dikaryotic mycelium of P. pulmonarius and monokaryotic mycelium of P. pulmonarius and vise versa were investigated, a hybrid with highest yield and brittle fruit body was HDS8. Yield harvested for 35 days. Backcross by di-mon crossing method was observed. Monokaryotic mycelium from five selected high yield hybrids were used. Dikaryotic mycelium from five hybrids and both parents (Pleurotus sajor-caju and P. pulmonarius) were used. There were four hybrids that gave high yield with better quality of fruit bodies (DSX15, PY3, SY2 and SB6). Two hybrids (SB6 and SY2) gave higher yield than the average of both parents by 53.7% and 46.9% respectively. Fruit body formation ability could be selected by cultured dikaryotic mycelium in the test tube containing sterilized sawdust. There was no relationship between mycelium growth rate and fruit body yield. Thirty five days harvesting period was enough to indicate the economical yield of the hybrids. Esterase isozyme patterns of mycelium from and 17 hybrid strains were investigated. They had a very close enzymic bands which were not able to be used to compared the differences. From the abovementions, interspecific breedings between Pleurotus sajor-caju and P. pulmonarius were possible both in the form of mon-mon, di-mon and backcross as di-mon crossing. Thus, they are belong to the same species of Pleurotus sajor-caju